Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers.

TLDR: None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation, and PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.

Abstract: A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L cv Kaghzi Kalan) using nodal explants The maximum shoot regeneration was observed on low level of BAP (01 mg l−1) or kinetin (05 mg l−1) BA was recorded to be better than kinetin in terms of number of days taken to bud break The maximum number of shoots per explant was observed on 01 mg l−1 BA and 05 mg l−1 kinetin Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (01 mg l−1 each), it increased with increasing concentration of BA up to 100 mg l−1 None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation In the case of a combination of BA + kinetin + IBA, the maximum (55 shoots per explants) proliferation was observed on MS medium containing 10 mg l−1 BA + 05 mg l−1 kinetin + 05 mg l−1 IBA or 025 mg l−1 BA + 10 mg l−1 kinetin + 10 mg l−1 IBA Regenerated shoots showed root induction on MS basal medium or on MS medium containing 10 mg l−1 IBA It is concluded that a five-fold increase (10 mg l−1 BA + 05 mg l−1 kinetin + 05 mg l−1 IBA) in axillary shoot proliferation, while seven-fold increase (025 mg/l mg l−1 BA + 10 mg l−1 kinetin + 10 mg l−1 IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication