Figure 1. The general vaccination strategy using an oxycodone hapten-immunoconjugate vaccine. A) The general vaccine strategy and B) scheme of Oxy hapten synthesis and preparation of Oxy-TT and Oxy-BSA immunoconjugates. C) Surface plasmon resonance (SPR) analysis of binding interactions confirmed that the polyclonal response presented binding to free drug in Cohorts 1 and 2. D) The vaccination schedule resulted in plasma anti-Oxy antibody midpoint titers from Oxy-TT vaccinated rats. Cohort 1 and Cohort 2 are separated into Upper or Lower groups (N=5-6) with alum and CpG ODN 1826 as adjuvants as determined by ELISA. Arrows indicate when rats were vaccinated. Data are presented as means ± SEM. Plasma from control TT vaccinated rats with alum and CpG ODN 1826 as adjuvants did not contain any detectable anti-Oxy IgG titers. TW and PK indicated tail-withdrawal tests and blood-draws for pharmacokinetic analysis, respectively.
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