Protein, immobilized on carrier by chemical binding method and capable of exhibiting aequorin activity

TLDR: In this paper, an organic or inorganic carrier having the ability to bind to a protein (eg apoaequorin) is swelled in a buffer solution and the swollen carrier is then suspended in a solution containing the protein having aequorin activity and reacted at 37 degC for 30min after completing the reaction, centrifugation is carried out to separate the resultant mixture into the carrier and a supernatant.

Abstract: PURPOSE:To obtain the subject immobilized protein, readily recoverable after use and useful as a biosensor, etc, by chemically binding a luminous protein having aequorin activity to an organic or inorganic carrier having the ability to bind to the protein CONSTITUTION:An organic or inorganic carrier (eg cellulose diethylaminoethyl ether) having the ability to bind to a protein (eg apoaequorin) is swelled in a buffer solution and the swollen carrier is then suspended in a solution containing the protein having aequorin activity and reacted at 37 degC for 30min After completing the reaction, centrifugation is carried out to separate the resultant mixture into the carrier and a supernatant and the carrier is subsequently washed with the buffer solution to afford the objective immobilized protein having the aequorin activity Furthermore, a protein, having the aequorin activity, obtained by cloning cDNA of a luminous jellyfish and prepared according to a technique of recombinant DNA is preferably used as the protein having the aequorin activity