Journal ArticleDOI
Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant.
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Single medium-based efficient protocols for large-scale multiplication of the rare woody aromatic medicinal plant Rotula aquatica Lour by means of axillary bud multiplication and indirect organogenesis were established using Murashige and Skoog medium to make the protocol economically advantageous.Abstract:
Single medium-based efficient protocols for large-scale multiplication of the rare woody aromatic medicinal plant Rotula aquatica Lour. by means of axillary bud multiplication and indirect organogenesis were established using Murashige and Skoog (MS) medium. There were no significant differences with respect to the induction of shoots per node or callus and roots per shoot on media prepared either with tap water and commercial sugar or those prepared with double distilled water and tissue culture-grade sucrose. The most effective medium for axillary bud proliferation was MS medium fortified with 1.0 mg l–1 N6-benzylaminopurine (BAP) and 0.5 mg l–1 indole-3-butyric acid (IBA), on which shoots were induced at the rate of 15 per node. The excision of node segments from the in vitro-derived shoots and their subsequent culture on medium supplemented with same concentrations of BAP and IBA facilitated enhanced axillary bud proliferation. Callus that developed from the lower cut end of the node explants induced shoots during subculture on half-strength MS medium with 1.0 mg l–1 BAP and 0.5 mg l–1 kinetin. The shoots developed rooted best on half-strength MS medium supplemented with 0.5 mg l–1 naphthaleneacetic acid (NAA). Rooted shoots, following acclimation in the greenhouse, were successfully transferred to field conditions, and 80% of the plantlets survived. When the basal ends of shoots harvested from multiplication medium were dipped in an NAA (0.5 mg l–1) solution for 25 days, a mean of 5.6 roots per shoot developed; the transfer to small pots facilitated the survival of 75% of the rooted shoots. Ex vitro rooting by direct transfer of the shoots from the multiplication medium to the greenhouse resulted in a 65% survival. Commercial sugar and tap water and ex vitro rooting make the protocol economically advantageous. About 750 plantlets were procured in a 3-month period starting from a single node explant.read more
Citations
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Journal ArticleDOI
Conservation in vitro of threatened plants - progress in the past decade
Viswambharan Sarasan,Ryan Cripps,Margaret M. Ramsay,Caroline Atherton,Monica Mcmichen,Grace Prendergast,Jennifer K. Rowntree +6 more
TL;DR: Photautotrophic micropropagation has the potential for development as a routine method for the in vitro conservation of endangered plants and the potential applications of cryopreservation are significant in this area.
Journal ArticleDOI
Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)
TL;DR: An effective pistachio (Pistacia vera L.) micropropagation system was developed involving rapid axillary bud proliferation and ex vitro rooting, which generated an optimal number of shoots with suitable morphological features.
Journal ArticleDOI
In vitro and ex vitro rooting of Siratia grosvenorii, a traditional medicinal plant
TL;DR: Ex vitro rooting technique was found superior to the in vitro one as plantlets developed through this method had lateral roots without any callus at the base of microcuttings, just like the natural root system and of course with higher root length, rooting rates, and transplant survival rate compared to theIn vitro developed plantlets.
BookDOI
Medicinal and Aromatic Plants of the World
TL;DR: MAPs have become “industrial products” with new concepts like phytotherapy and veterinary medicinal uses, aromatherapy, nutraceuticals, cosmeceuticals, and animal welfare uses widening the scope of the utilization.
Journal ArticleDOI
Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis
TL;DR: An efficient in vitro propagation system has been developed for rapid micropropagation of Soapnut, a medicinally and economically important tree from nodal (axillary bud) segments of seedlings, which can be applied for further genetic transformation assays and pharmaceutical purposes.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book
Plant Propagation by Tissue Culture
TL;DR: The Anatomy and Morphology of Tissue Cultured Plants M.V. Moshkov, G. V. Novikova, M. Stasolla, E. Zazimalova and E.F. George reveal the secrets of successful tissue culture and the challenges faced in implementing and sustaining such a system.
Book
Ayurvedic Drugs and Their Plant Sources
TL;DR: Ayurvedic Drugs and Their Plant Sources, Ayurveda Drugs and their Plant Sources and their plant sources, مرکز فناوری اطلاعات و کسورزی.
Journal ArticleDOI
In vitro micropropagation of Gymnema sylvestre - a multipurpose medicinal plant.
TL;DR: The nature of the explant, seedling age, medium type, plant growth regulators, complex extracts and antioxidants markedly influenced in vitro propagation of Gymnema sylvestre and the plantlets were hardened and successfully established in natural soil.
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