Spatial separation of parental genomes in hybrids of somatic plant cells.
TLDR
The degree of genome separation calculated for different cell clones remained constant during in vitro propagation of cells but was significantly lower for subclones derived from colchicine-treated cells, concluding that spatial chromosome arrangement in metaphase is epigenetically controlled.Abstract:
Chromosome spatial arrangements on metaphase plates of intergeneric intertribal cell hybrids of Nicotiana chinensis and Atropa belladonna as well as interspecific somatic hybrid plants of Nicotiana plumbaginifolia and Nicotiana sylvestris were analyzed. In the metaphases of the first divisions of protoplast fusion products, chromosomes of the two parents were spatially separated (segmented metaphase). In long-term cultured somatic hybrids, the topology of genome separation pattern in both callus cells and plants showed changes in form from “segmental” to “radial.” Growing the hybrid cells in the presence of colchicine resulted in random chromosome arrangement both in cells directly exposed to different colchicine concentrations and in colchicine-treated cells grown in colchicine-free media. The degree of genome separation calculated for different cell clones remained constant during in vitro propagation of cells but was significantly lower for subclones derived from colchicine-treated cells. Therefore, it is concluded that spatial chromosome arrangement in metaphase is epigenetically controlled.read more
Citations
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In Situ Localization of Parental Genomes in a Wide Hybrid
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The initiation of meiotic chromosome pairing: the cytological view.
TL;DR: The present paper reviews the controversial cytological evidence, hypotheses, and ideas on how the first contact between homologous chromosomes comes about.
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Parental genomes are separated throughout the cell cycle in a plant hybrid
TL;DR: Results show that the nucleus of the mature, sexual F1 hybrid plant Hordeum vulgare is spatially organized above the level of the DNA and chromosome at the genome level.
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Investigations of genome relationships between Leymus, Psathyrostachys and Hordeum inferred by genomic DNA:DNA in situ hybridization
M. Ørgaard,J. S. Heslop-Harrison +1 more
TL;DR: Genome relationships between the genera Leymus Hochst.
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Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization.
TL;DR: Genomic in situ hybridization to plant chromosomes provides a rapid and reliable means of screening for recombinant genotypes in asymmetric somatic hybrids and contributes to a greater understanding of the events responsible for genomic recovery and restabilization following genetic manipulation in vitro.
References
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Journal Article
Hordeum and secale mitotic genomes lie apart in a hybrid
TL;DR: In both unpretreated root tip metaphases and pretreated mitoses of Hordeum vulgare L. cv.
Journal ArticleDOI
Identical Linear Order of Chromosomes in Both Gametes of the Acoel Turbellarian Polychoerus carmelensis: A Preliminary Note
TL;DR: In an exceptionally well-defined first cleavage metaphase in a sectioned egg of Polychoerus carmelensis all 34 (2n) chromosomes were present in a single 8-micron section, and it was possible to identify the homologous chromosomes derived from the two parent gametes.