Showing papers in "Plant Science in 1989"

TL;DR: Preliminary analysis of the genetic behaviour of the myc− mutants in diallel crosses has shown that at least three genes are involved in VA mycorrhiza infection.

TL;DR: Grafting experiments showed the (nod − ) and ( nod + , fix − ) phenotypes are associated with the root genotypes and that (nad ++ , nts) phenotype is associated withThe shoot genotype.

TL;DR: It is shown that selection and medium-composition are essential factors in obtaining good growth and scopolamine production in hairy root clones established by culturing hairy roots for at least 5 passages in phytohormone-free HF medium.

TL;DR: Tolerance of desiccation was induced in alfalfa somatic embryos by exogenous application of abscisic acid, but some of these stress pretreatments had other deleterious effects on embryo maturation and seedling vigor after imbibition.

TL;DR: A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris ssp.

TL;DR: Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing β-glucuronidase (GUS) transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S) or a class-I patatin gene.

TL;DR: Sexually-mature dry bean plants (Phaseolus vulgaris L.) were regenerated from cotyledonary node tissue of seedlings germinated on Murashige and Skoog (MS)-based media supplemented with 5 μM benzyladenine (BA).

TL;DR: It is concluded that the Glu-1Ay gene is transcriptionally inactive and is silenced by mutations in the 280 bps immediately upstream of the transcription start site and not by the 85-bp deletion or any other changes further upstream.

TL;DR: Embryogenic calli were initiated from immature zygotic embryos of hybrid larch dissected at the precotyledonary stage and influenced by the type and concentration of the gelling agent.

TL;DR: The developmental stage of the zygotic immature embryos, genotype and culture medium are critical factors for the production of embryogenic callus and the origin of somatic proembryos in Pinus elliottii was from single cells of the suspensor cell callus.

TL;DR: It is suggested that auxin-induced ethylene biosynthesis plays an important role in the development of somatic embryos in carrot, and the promotion of polyamine biosynthesis (by DFMO in the present case) may cause a reduction in the cellular pools of S -adenosylmethionine, thus allowing embryogenesis to occur in the presence of an auxin.

TL;DR: It is suggested that ABA or osmotic conditions influence the distribution of fatty acids between the pathways leading to desaturation or elongation, but some other factor(s), not duplicated by the culture treatment, control the availability of substrate for these pathways.

TL;DR: Southern blot analysis of the GBSS genes from both potato genotypes revealed that the amylose-free mutant, which lacks GBSs activity and protein, does not contain a large structural lesion in the GbsS gene.

TL;DR: Apical and axial shoot buds of Valeriana uallichii DC, an endangered medicinal plant, were successfully encapsulated in calcium alginate beads and plantlet development was observed under both in vitro and in vivo conditions.

TL;DR: Polygalacturonase was found in pollen of the twelve monocotyledon species examined, and can be classified as an exopolygalaturonase, which is most effective on galacturonans with moderate chain lengths.

TL;DR: The JA-dependent increase in leaf storage proteins suggests that JA may play a role in the regulation of the amount of leaf nigrogen that partitions into VSP, possibly under stressed conditions.

TL;DR: The induction of β-1,3-glucanase and chitinase induced by P. tabaci and P. parasitica are the same basic isoforms known to be induced by tobacco mosaic virus, therefore the induction appears to be a non-specific response of the host to pathogens.

TL;DR: Various inoculation procedures of in vitro grown wheat seedlings with Agrobacterium tumefaciens carrying a wheat dwarf virus (WDV) dimer led to 13–46% agroinfection.

TL;DR: Different vegetative parts of Citrus mitis seedlings and mature plants were used as explants in culture and buds were obtained from intact roots on whole seedlingx cultured in medium with 0.5 mg 1−1 BAP.

TL;DR: In the presence of S-adenosyl-l-methionine, enzyme preparations from carrot (Daucus carota L.) cell suspension cultures specifically catalyze the methylation of caffeoyl-CoA to feruloysl- CoA indicating that feruloyl -CoA is an intermediate in the defence reaction of the cells.

TL;DR: Pollen and stigma contain vir inducing compounds, which are released in high concentrations only by pollen, and such flavonoids could play a more important role in the natural infection of plants by Agrobacterium than acetosyringone, which until now has been found only in tobacco.

TL;DR: The results show that all of the constituent cells of the isolated corn embryo sac are structurally intact, viable easily identified, and contain only remnants of a cell wall.

TL;DR: A simple method for the Agrobacterium-mediated transformation of callus cultures of nine plant species, Lycopersicum esculentum Mill, Petunia hybrida Vilm, Pimpinella anisum L, Solanum melongena L, Nicotiana glauca Graham, N. glutinosa L., N. plumbaginifolia Viviani and N. tabacum L., is described.

TL;DR: It is pointed out that culture conditions are fundamental to obtain the highest transformation efficiency in potato and not just theoretical cultivation conditions.

TL;DR: Large numbers of asymmetric nuclear hybrids have been obtained by fusion between protoplasts from nitrate reductase deficient mutants of Nicotiana plumbaginifolia and gamma-irradiated protoplast ofNicotiana sylvestris to study the elimination of donor genetic material as a function of radiation effects and in vitro passage.

TL;DR: The results indicate a close relationship between changes in plant growth and morphology in response to altered light quality, mediated via the photoreceptor, phytochrome, and altered enzyme activity in both source and sink organs.

TL;DR: With few exceptions, the capacity to regenerate green shoots decreased with suspension culture age and was totally lost on average 25 weeks after suspension culture initiation, and protoplasts formed numerous microcalli in liquid and agarose solidified media.

TL;DR: The age of the embryo was an important factor determining callus proliferation and subsequent embryogenesis and more than 50% of embryos excised from 6–7-month-old nuts produced embryogenic callus tissues.

TL;DR: Primary crown gall tumours induced on stems on tomato plants by Agrobacterium tumefaciens yielded extracts with pronounced cytokinin activity and 6-benzylaminopurine and its 9-β-ribofuranoside were identified as cytokinins in the extracts.

TL;DR: The results from this study point to weak but distinct embryo specific expression of gene(s) governing the biosynthesis of petroselinic acid in later stages embryoids but not in undifferentiated tissues.