Study on the extraction of the total alkaloids from Caulopyhllum robustum

TLDR: This technology is simply, cheap effective and feasible for manufacture in great scale, and the optimized conditions for purification using cation exchange resin were investigated.

Abstract: OBJECTIVE To study the technological parameters of the extraction process of the total alkaloids from Caulopyhllum robstum. METHODS Taspine, whiVh is main component of the total alkaloids from Caulopyhllum robustum, was selected as an evaluating marker and determined by HPLC. The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated. RESULTS The optimized conditions in the process of acid water extraction were 1% hydrochloric acid as much as seven times of the medicine amount for 24hs and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin LSD001 at 2 ml/min of flow rate, then eluted with 10BV of 4% aqueous ammonia ethanol. The extraction ratio of the total alkaloids was 1. 35% and the content of taspine of the total alkaloids was 6. 80%. CONCLUSION This technology is simply, cheap effective and feasible for manufacture in great scale.