Showing papers in "Chinese Journal of Experimental Traditional Medical Formulae in 2012"

Suggestion on Selection of Chinese Patent Medicine Lists Recorded in Pharmacopoeia of People's Republic of China Volume I(2015 Version)

Abstract: Make a suggestion concerning the selection of Chinese Patent Medicine lists recorded in pharmacopoeia of the people's republic of China volume I(2015 version).Analyzing Chinese Patent Medicine lists which contains endangered species and National Protected animals strain,recorded in pharmacopoeia of the people's republic of China volume I(2010 version);Analyzing Clinical Medicine lists and Minority Medicine lists recorded in pharmacopoeia of the people's republic of China volume I(2010 version).The Selection principles and the re-estimation of Species in pharmacopoeia of the people's republic of China volume.The proportion of the selection of Clinical medicine.The selection of medicine which contains endangered species and National Protected animals strain.The selection of national medicine.The selection of medicine species has been disconnected with the application of basic medicine,medicare medicine and endangered species.Expect to establish the catalogue of selected medicine concerning the pharmacopoeia of the people's republic of China.Make definite and clear of the selection principles of medicine specie,which should conclude the basic medicine and medicare medicine as well.Adjust the proportion of clinical medicine,in order to fulfill the demand of clinical work.The selection of medicine which contains endangered species should obey the law of nation.Acquire more application of traditional national medicine.Confirm more communication and combination with the related Department of medicine in China.

Study on Antidepressant Effect and Mechanism by Cyperus rotundus Extracts

Abstract: Objective:To screen the antidepressant fractions from Cyperus rotundus and investigate the possible a mechanism.Method: The extract and florentine were orally given to mice at a dosage of 10 g·kg-1 and 0.08 g·kg-1 per day,respectively.The swimming test and the tail suspension test were applied to observe the antidepressant effect of C.rotundus extracts.The content of serotonin(5-HT),dopamine(DA) and noradrenalin(NE) was determined by RP-HPLC method to study the mechanism of C.rotundus extracts.Result: The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus significantly shortened immobility time in swimming test and the tail suspension test.The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus markedly increased the content of 5-HT and DA with no action of NE.Conclusion: The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus can produce an antidepressant effect,the mechanism of which may be related to affecting the 5-HT and DA nervous system.They may be the active part of C.rotundus.

Content Changes of 5 Components in Polygohum muhiflorum During Processing

Abstract: Objective:To study the content changes of gallic acid,5-hydroxymethyl furfural,emodin,2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside and emodin monomethyl ether in Polygohum muhiflorum during processing and establish a method of determination by HPLC.Method: The separation was performed on a KinetexTM C18 column(4.6 mm×100 mm,2.6 μm),using acetonitrile and water(0.5% acetic acid pH 2.9)as the mobile phase with gradient elution.The flow rate was 1.8 mL·min-1;column temperature was at 30 ℃ and the detection wavelength was 254,270,284,320 nm,respectively.Result: The linear ranges of gallic acid,5-hydroxymethylfurfural,emodin,2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside and emodin monomethyl ether were 0.585-58.5,0.54-53.5,1.07-107,1.23-123,0.564-16.92 mg·L-1,respectively.The content of gallic acid was increased significantly and 5-HMF was a new component after processing,and timing had a little effect on the content change of gallic acid,however,the content change of 5-HMF was higher and higher in the process of time till 72 h.Meanwhile,the content of stilbene glycoside was decreased in the process of time during processing and dissociative anthraquinone,emodin and emodin monomethyl ether,both of them were increased by processing after 32 h the content reach the maximum.Conclusion: The method is simple,accurate and can be used for quality control of P.muhiflorum.Further research of the correlation between the content changes of these 5 components in P.muhiflorum and pharmacodynamics as well as dose-effect relationship should be done.

Advance in Study on Compound Kushen Injection

Abstract: According to the literatures on the study of Compound Kushen Injection published in recent years.The advance on its chemical constituents,quality control,drug metabolism,pharmacological activities and clinical applications were summarized.The main constituents of Compound Kushen Injection are alkaloids,including oxymatrine and matrine,and the injection has the diverse activities of anti-tumor,analgesia and raising tumor patient's immunity.It was widely used for the adjuvant therapy of end-stage cancer.It should be further investigated for the quality consistency and the effectiveness as well as the safety of the product.

Development and Application of Traditional Chinese Medicine Inheritance Support System

Abstract: Objective:We developed traditional Chinese medicine inheritance support system on the basis of the comprehensive and objective analysis of pattern and features of succession of Chinese medicine.Method:It concludes Chinese medical diagnosis and treatment information collection module of for clinical data,knowledge management and service module for ontology of Chinese medicine and potential knowledge mining module for inheritance and innovation individually to effectively solve the problems of non-standardization and individuation,establishing a self-service platform based on the thinking of satisfying individual demand,taking methods and techniques of artificial intelligence,data mining,network science and other disciplines,supplying the core demands of inheritance,development,dissemination and innovation in clinical diagnosis and treatment and inheritance of traditional Chinese medicine.Result:This system could improve the applications of experience succession of rich experiential doctors,individual experience conclusion of physicians,and study of young physicians and prescription discovery of new Chinese medicine.Conclusion:It enriched inheritance model and effectively satisfied the demand of inheritance and development of Chinese medicine.

Effect and Mechanism of Shenling Baizhu San on the Murine Model of Inflammatory Bowel Disease Induced by Dextran Sodium Sulfate in Mice

Abstract: Objective:To investigate the effect and mechanism of Shenling Baizhu San on the murine model of inflammatory bowel disease(IBD) induced by dextran sodium sulfate.Method: IBD was induced by taking the drinking water added 5% dextran sodium sulphate(DSS) for 7 days.The BALB/c mice of different groups were ig administrated with normal sodium,5-aminosalicylic acid(5-ASA),Shenling Baizhu San(12,6,3 g·kg-1),respectively.Body weight,feces description,hemafecia,disease activity scoring(DAI),reactive oxygen species(ROS),myeloperoxidase(MPO),content of malondialdehyde(MDA) in bowel tissue and histological scoring,and expression of TNF-α,IL-1β,IL-6 protein were observed.Result: Levels of ROS,MPO and MDA in DSS induced group were increased significantly and expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6) protein were also increased.Three doses of Shenling Baizhu San could decrease ROS,MDA and MPO content,down-regulate TNF-α,IL-1β,IL-6 protein expression.Conclusion: Shenling Baizhu San can protect the damage in DSS-induced IBD in mice,which may be related to regulating inflammatory factor and scavenging oxygen free radicals.

Dose-toxicity Relationship Study for Cardiotonic Effect of Aconitine

Abstract: Objective:To analyze the relationship between toxicity and cardiotonic effect of aconitine,calculating the therapeutic index(TI) of aconitine.Method: Regarding rat's electrocardiogram(ECG) change as the index,the toxicity to heart induced by aconitine was studied.Nimodipine,one of Ca2+ antagonist,was used to make heart failure model in rats.The aconitine was injected through femoral vein continuously.The effect on heart rate,+dp/dtmax,LVDP-rate pressure product(RPP) was observed.Result: Regarding RPP or +dp/dtmax as the index of ED50 and regarding PVC or CA as the index of TD50 or LD50,LD50/ED50 or TD50/ED50 was used to calculate the therapeutic index(TI) of aconitine,TI(RPP)were 1.84,17.22 and TI(+dp/dtmax)were 1.23,11.54 respectively.Conclution: Aconitine has cardiotonic effect but has toxicity to heart.TI is small.

Pharmacodynamic Compariason of Fritillaria unibracteata,Fritillaria unibracteata var.wabensis and Fritillaria thunbergii Cultivated

Abstract: Objective:Experiments were carried on to compare the antitussive,expectorant and antiasthmatic effects of Fritillaria unibracteata,Funibracteata varwabensis and Fthunbergii and identify if the Funibracteatawabensis can be used as the alternative resources of wild FcirrhosaMethod:The antitussive effects of the 3 kinds of fritillarias s were investigated through testing the cough times and EDT50 induced by ammonium hydroxide in mice and the counts of cough induced by critic acid in guinea pigsThe expectorant activities were sdudied by testing the amount of phlegm secreted in mice and ratsThe antiasthma effect was studied by the asthma time induced by acetylcholine chloride and histamine phosphate in guinea pigsThere were negative control,positive control and 3 kinds of fritillarias groups in every experimentThe dose of fritillarias in the antitussive experiment was 25 g·kg-1·d-1in mice and 15 g·kg-1·d-1in guinea pigsThe dose of 3 fritillarias in the expectorant experiment was 2 g·kg-1 ·d-1in mice and 1 g·kg-1·d-1 in rats,The dose of 3 kinds of fritillarias in the anti-asthma experiment was 15 g·kg-1·d-1The administration time of every experiments was 5 daysResult: Funibracteata varwabensis cultivated has similar eliminating phlegm activities and antiasthma effects to FunibracteataIt also has similar antitussive effects to Funib-racteata and Fthunbergii MiqConclusion:Funibract-eata varwabensis cultivated can be used as alternative resources of wild Fcirrhosa

Study on Flavonoids Constituents of Blumea balsamifera

Abstract: Objective:To study the flavonoids constituents of the aerial parts of Blumea balsamifera.Method:The compounds were isolated and purified by silica gel,Sephadex LH-20,medium pressure column chromatography,and their structures were elucidated by spectral methods and chemical analysis.Result:Twelve compounds were isolated and their structures were identified as 3,3',5,5',7-pentahydroxyflavanone(1),3,3',4',5-tetrahydroxy-7-methoxyflavanone(2),chrysoeriol(3),3',4',5-trihydroxy-3,7-dimethoxyflavone(4),diosmetin(5),3,3',4',5-tetrahydroxy-7-methoxyflavone(6),3,5-dihydroxy-3',4',7-trimethoxyflavone(7),chrysosplenol C(8),3,3',5-trihydroxy-4',7-dimethoxyflavanone(9),blumeatin(10),3,3',5,7-tetrahydroxy-4'-methoxyflavanone(11),3',5,5',7-tetrahydroxyflavanone(12).Conclusion: Among them,compounds 1,3-5 and 8 were isolated from the genus Blumea for the first time,and compounds 6,7 were isolated from B.balsamifera for the first time.

Experimental Study on Antihypertension and in vivo Antioxidant Function of Coreopsis Extract

Abstract: Objective:To study the influence of Coreopsis extract eluted by 50% ethanol on antihypertension and in vivo antioxidant function of hypertension miceMethod: Hypertension model of mice were induced by giving high salinity water continuously by gastric irrigation(ig) and keeping them in low-temperature environment 4 hours a day for 20 daysThen the mice were given drug continuously by ig for 28 days,measured blood pressure,collected blood and liver,and detected the activities of SOD,MDA in serum;meanwhile the livers were sampled for measurement of MDA content,activities of SOD,GSH-Px and T-AOCResult: Compared with blank group,the blood pressure of control group was depressed significantly;SOD,GSH-Px and T-AOC activities of control group both in serum and livers were reduced significantly,results above indicated that the model was set up successfullyThe drug can depress blood pressure,increase SOD,GSH-Px and T-AOC activities,reduce the content of MDA compared with control groupConclusion: Coreopsis extract can significantly depress blood pressure,increase the ability of antioxidant effect of hypertension mouse in vivo,and reduce oxidation injury caused by hypertension,protect blood vessel and internal organs had been injuriedAll results indicated that it is one of the mechanisms of antihypertensive effects of Coreopsis extract

Effcet of Effective Parts from Musa basjoo on Blood Glucose and Glucose Tolerance in Mice

Abstract: Objective:To study the effect of effective parts from Musa basjoo(EPM) on blood glucose and glucose tolerance in mice.Method: Diabetic model was induced by intraperitoneal injection of Alloxan once.Diabetic mice were randomly divided into model group,Metformin group and high,middle and low dose of EPM groups(200,100,50 mg · kg-1).All groups were fed for 14 consecutive days,and then the blood glucose was detected on the fifteenth day.Effect on the glucose tolerance in model and normal mice were detected at the same time.Result: EPM could significantly decrease the blood glucose level of diabetic mice(P0.05)and ameliorate the glucose tolerance in normal and diabetic mice(P0.05),but it had no significant effect on the blood glucose level of normal mice.Conclusion: EPM can significantly decrease the blood glucose level in diabetic mice and ameliorate the glucose tolerance in normal and diabetic mice.

Study on Spectrum-effect Relationship betweent Fingerprint of Essential Oil and of Anti-tumor Effect from Curcuma kwangsiensis

Abstract: Objective:To investigate the effective component group represented by fingerprint contributed to pharmacodynamic by grey relational analysis,and provide a reference for traditional Chinese medicine quality control and develop evaluation criteria of pharmacodynamics.Method: The fingerprint of essential oil from Curcuma kwangsiensis was established by gas chromatography-mass spectrometry(GC-MS),and the anti-tumor effect of curcuma oil was studied.At the premise of the acquired GC-MS characteristic of fingerprint and pharmacodynamic data to inhibiting proliferation of nasopharyngeal carcinoma cell,the relational grade and relational sequence between fingerprint of essential oil and of inhibiting proliferation rate were calculated by grey relational analysis.Result: According to the relational grade the sequence of contribution to inhibiting proliferation to nasopharyngeal carcinoma cell was curcumolcurdioneβ-elemenegermacronecurzerenoneα-caryophylleneδ-selineneβ-caryophylleneβ-elemenonea-pineneα-caryophylleneeucalyptolcamphor2-nonanolisoborneolcurzereneδ-elemenecampheneD-limoneneborneol.Conclusion: The anti-tumor effect of C.kwangsiensis is the result of combined effect of 'effective component group.This study identifies the effective component group represented by fingerprint contributed to pharmacodynamic,and provides a reference for establishing fingerprint of traditional Chinese medicine's spectrum-effect relationship,traditional Chinese medicine quality control and develop evaluation criteria of pharmacodynamics.

Screening of Antidepressant Effects of Four Main Flavonoids Compounds from Scutellaria baicalensis

Abstract: Objective:To screen antidepressant activity of baicalin,baicalein,wogonoside and wogonin,the flavonoids from Scutellaria baicalensisMethod: Male ICR mice were randomly divided into 10 groups:control group,fluoxetine group and baicalin,baicalein,wogonoside and wogonin groupsThe treatment groups were received four flavonoids at dose of 10,20 mg·kg-1 and fluoxeine at 20 mg·kg-1 for 7 days respectivelyForced swimming test(FST),tail suspension test(TST) and open field test(OFT) were applied to evaluate antidepressant activity at 1 hour after the last administrationResult: Compared with control groups,the immobility time of FST mice was significantly shorten from(1076±285) s to(644±251),(732±308),(696±204),(797±326) s,following treatment at dose of 20 mg·kg-1,with baicalin,baicalein,wogonoside and wogoninMoreover,the immobility time of TST mice was also significantly shorten from(852±273) s to(431±164),(520±211),(483±259),(622±358) s,respectivelyParticularly,the effects of baicalin and wogonoside were more obviousIn addition,four flavonoids had no effect on spontaneous locomotor activity in miceConclusion: Our results suggest that all of four flavonoids compouds from Sbaicalensis exhibit antidepressant activityMoreover,the roles of baicalin and wogonoside were more visible than baicalein and wogonin,which may be relate to the different metabolic processes of these compouds

Advances of Pharmacological Researches in Constipation Animal Model

Abstract: The methods of building constipation models were analyzed and summarized completely.With constipation,animal models as key words,China Academic Journal Network Publishing Database and Springer Link were retrieved.Relevant 86 references were get.24 articles for reference were adopted through screening.This paper reviewed the methods of building constipation models in the rats,mice,monkeys,rabbit and ferret,and summed up the characteristics and applicable range of each method.The making methods of constipation model were various,but that methods and observation index of constipation models were not in-depth.Detection technology of modern biochemical,molecular and cellular should be used for exploring constipation mechanism.The design thoughts of the models should conform to clinical constipation pathogenesis,and then the evaluation standards of specific constipation model should be found,that can provide convenience for the innovative research of constipation models and research and development of treating constipation medicines.

Absorption and Metabolism of Chlorogenic Acid

Abstract: To summarize chlorogenic acid in absorption site and mechanism,metabolic site and pathways,and effect on pharmcokinetic parameters of chlorogenic acid with crude drugs compatibility.Recent relevant information and references were summarized,and further analyzed and reviewed.The current study showed that chlorogenic acid could be absorbed in the stomach,small intestine and large intestine,which was absorbed into the bloodstream in the original form by the stomach and small intestine.In addition,the absorption of chlorogenic acid was also affected by fasting or satiety.The main metabolic part of chlorogenic acid was in the intestine,as well as some in the liver.Chinese compatibility can promote the absorption and distribution of chlorogenic acid in the body in order to quickly reach peak concentration.We should carry out further studies on the main absorption site,mechanism of absorption in the stomach,the role of the metabolites of chlorogenic acid on its activity,and provide useful references to understand the process of chlorogenic acid in vivo,explore the association between absorption and metabolism with activity,and study pharmacokinetic.

Simultaneous Determination of Piperine and Piperlongumine in Piper longum by HPLC

Abstract: Objective:To establish a high performance liquid chromatographic method(HPLC) for sim ultaneous determination of piperine and piperlongumine in Piper longum.Method:Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) was used to separate the analytes using a mode with a mobile phase consisting of water with 0.25% formic acid(35) and methyl alcohol(65) at a flow rate of 1 mL·min-1 and an operating column temperature of 40 ℃.The detection wave length was set at 343 nm.Result:All the constituents showed good linearity(r=0.999 9) in the range of the tested concentration,the average recoveries of the method were 100.4% and 102.2%,RSD were 2.6% and 2.5%.This method successfully determined 2 compounds in P.longum(from 8 different origins) simultaneously and quantitively.Conclusion:This is a more reasonable and credible quality control method for the Chinese traditional medicine of P.longum and it is simple,well reproducible.

Characterization on Central Ingredients of Active Components in Dachuanxiong Fang based on HPLC-DAD-MS~n

Abstract: Objective:To investigate central ingredients of active components in Dachuanxiong fang(DF) by HPLC-DAD-MSn,so that the quality of DF as well as its preparations can be effectively and systemically controlled and the substance basis of DF can be explored.Method: HPLC fingerprint spectrum of the active parts of Dachuanxiong fang was established.HPLC-DAD-MSn was developed for identification of the active parts of Dachuanxiong fang.Result: Three components of the active parts of chuanxiong and 8 components of the active parts of tianma were identified.Conclusion: Research on central ingredients of active components in Dachuanxiong fang is favorable to the clarification of its active substance basis and perfection of quality stantard.

Research Progress of Endothelial Dysfunction and Atherosclerosis

Abstract: This paper presented the reseach progress of the relationship between vascular endothelial dysfunction and atherosclerosis via literature review and analysis on therapeutic regulation on vascular endothedial dysfunction for treatment of atherosclerosis using traditional Chinese medicineThrough the keywords of vasomotor,endothelial dysfunction,atherosclerosis,etcthirty eight literature introducing the relationship between endothelial dysfunction and atherosclerosis,and summing up the research progress of treatment of endothelial dysfunction in the recent decade was collected and reviewedThe methods of treating atherosclerosis through regulating vascular endothelial dysfunction were summarized generally as the complementary therapy of natural substances for restoration of endothelial function,endothelial plantation and gene therapy,exogenous nitric oxide(NO),endothelin antagonists,etc;the progress on treating atherosclerosis through regulating NO and endothelin(ET) using Chinese single herb and formulas was presentedThe results show that the methods of regulating the vascular endothelial dysfunction were diversified,the further study on the relationship between atherosclerosis and vasomotor substances such as NO/ET is the important research directionThe traditional Chinese medicine has significant role in the protection of endothelial dysfunction andregulating the endothelial secretion of active substances,but it lacks the depth on the material basis and mechanism that regulating vascular dysfunction,efficacy,so it will has broad prospects in the fields of preventing and treating cardiovascular disease to further explore the anti-atherosclerotic mechanisms of traditional Chinese medicine,playing the advantages of multi-target regulation of Chinese herbal compound,developing the anti-atherosclerosis traditional Chinese medicine based on the regulation of vascular dysfunction

Study on Antitumor Activity of Five Furanocoumarins from the Root Bark of Changium smyrnioides in vitro

Abstract: Objective:To study the in vitro activity of five furanocoumarins from the root bark of Changium smyrnioides Wolff.isoimperatorin,imperatorin,xanthotoxol,phellopterin and 5-hydroxyl-8-methoxy-psoralen.Method:The antitumor effect against human liver cancer(SMMC-7721 and HepG2),human lung cancer(A549),human gastric cancer(MKN-45),human uterine cervix cancer(Hela) and human breast cancer(MCF-7 and MDA-MB-231) was observed by MTT.Result:Five furanocoumarins had different inhibition on seven kinds of tumors.IC50 was from 0.30 mg·L-1 to 17.23 mg·L-1,especially isoimperatorin was most significantly effective.IC50 was from 0.39 mg·L-1 to 4.11 mg·L-1.Conclusion:These five furanocoumarins have obvious antitumor effect.

Effects of Centipede Extraction on vWF and TPO Expression in Blood Plasm after Focal Cerebral Ischemia-reperfusion Injury in Rats

Abstract: Objective:To investigate the effects of centipede extraction on von willebrand disease fector(vWF) and thrombopoietin(TPO) expression after focal cerebral ischemia-reperfusion injury in rats.Method:Intraluminal thread occlusion of the middle cerebral artery(MCAO) was applied to establish the focal cerebral reperfusion model.The rats were randomly divided into six groups: sham-operative group,model group,high dose of centipede extraction group,middle dose of centipede extraction group,low dose of centipede extraction group and Yiqi Huoxue Recipe(YHR) group.The rats were reperfused again after occlusion of 2 hours.After intragastric administration of different drugs for 14 days,the blood plasma vWF and TPO were measured by enzyme-linked immunosorbent assay(ELISA).Result:The vWF and TPO levels were significantly up-regulated in rats after ischemia-reperfusion injury compared with sham-operative group.Compared with the model group,high dose of centipede extraction significantly ameliorated the vWF and TPO expression(P0.05).Yiqi Huoxue Recipe administration could just decrease TPO level(P0.05).The middle dose and low dose of centipede extraction reduced vWF and TPO levels without significant difference compared with model group.Conclusion: Centipede extraction could ameliorate cerebral ischemia-reperfusion symptoms via down-regulating the vWF and TPO levels.

Antioxidation of Different Extracts from Isatidis indigotica in vitro

Abstract: Objective: Antioxidation of different extracts from Isatidis indigotica were studied in vitro to supply the separating base of antioxidative components.Method: The extracts were prepared by macroporous resin and anion/cation exchange resin.Their antioxidant activities were assessed by two complementary test systems,namely 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity and ferric reducing antioxidant power(FRAP) assay.Result: The total lignin displayed strong activity in all test systems.(DPPH IC50 160.6 mg·L-1;FRAP 0.78 mmol·g-1) Banlangen Keli(DPPH IC50 595.97 mg·L-1;FRAP 0.12 mmol·g-1) showed relatively lower activity.Total organic acids and total alkaloid have a little activity.Conclusion: Antioxidative components could be justified existing in the total lignin initially.

Antibacterial and Antiviral Effects of Strictosamide

Abstract: Objective:To observe the antibacterial and antiviral activities,and provide certain experimental data for strictosamide that is intended for upper respiratory tract infection.Method: The in vitro antibacterial activities on standard staphylococcus aureus and other 9 strains were observed by both test-tube and plating methods,and the protective effects on mice infected by staphylococcus aureus and streptococcus pneumonia were also observed.Mice were divided into 5 groups,such as normal group,model group,reduning group(crude drugs 8.7 g·kg-1),low and high dosage(20,40 mg·kg-1) of strictosamide groups.They were injected intraperitoneally once a day for 7 days.At 1 h after the last injection,staphylococcus aureus or streptococcus pneumonia was injected intraperitoneally to mice,and then the death of mice was observed within 7 days.The in vitro antiviral activities were observed on the model of MDCK cells infected by influenza A(A/PR8/34(H1N1)) and B(B/Jingfang 98-76) virus,and the in vivo antiviral activities were observed by the model of mice infected by influenza A virus.Mice were divided into 5 groups,such as normal group,model group,reduning group(crude drugs 8.7 g·kg-1),low and high dosage(20,40 mg·kg-1) of strictosamide groups.They were injected intraperitoneally once a day for 5 days,and virus was given to mice by nose on the first day.After the last injection,the lung lesions and pathological changes were observed,and the pulmonary index was also calculated.Result: Strictosamide had inhibiting effects on standard escherichia coli and other 6 strains in vitro with MIC of 5 g·L-1.At 10 g·L-1,the diameters of inhibiting bacteria circle of diplococcus pneumonia and other 4 strains were 13-25 mm.At 40 mg·kg-1,the survival rate of mice infected by staphylococcus aureus and streptococcus pneumonia was 70% and 55%,separately.Strictosamide showed inhibitory effects on both influenza A and B viruses in vitro with IC50 values of 0.649,0.323 g·L-1,respectively.It also significantly inhibited increased pulmonary index and improved pulmonary pathological lesions of the infected mice caused by influenza A virus,and the inhibitory rate of pulmonary index was 39.8% at 40 mg·kg-1.Conclusion: Strictosamide possesses antibacterial and antiviral activities.

Antidepressant Effect of Fingered Citron Volatile in Rats

Abstract: Objective:Previous studies have demonstrated that fingered citron volatile produced antidepressant-like action in various mouse models with behavioral despair.The present study aimed to examine whether fingered citron volatile could affect the chronic unpredictable mild stress(CUMS)-induced depression in rats.Method: Ninety male Wistar rats were randomly divided into six groups as follows: control,model,positive control(orally given fluoxetine 10 mg·kg-1 for 21 days),low,middle and high dosages of fingered citron volatile(orally given 150,300,600 mg·kg-1 of fingered citron volatile for 21 days).All groups except control were exposed to chronic stress for 21 days to establish depression model and every rat was given corresponding drugs simultaneously.After 21 days,the behavior changes were measured by sucrose preference and open-field,serum corticosterone level was measured by radio-immunity and brain-derived neurotrophic factor(BDNF) protein levels was measured by Western-blot.Result: CUMS being lasted for 3 weeks,caused depression-like behavior in rats,as indicated by the significant decrease in sucrose preference(from 88.1%±13.3% to 60.5%±9.8 %) and in open-field scores(from 123.5±21.3 to 38.2±5.9 and from 72.5±12.7 to 17.1±5.1,respectively).Whereas serum corticosterone level was significantly increased in rats exposed to CUMS [from(42.1±7.7) μg·L-1 to(117.6±19.3) μg·L-1],and BDNF protein expression were decreased(from 0.22±0.04 to 0.07±0.02) in CUMS-treated rats.Daily intragastric administration of fingered citron volatile(150,300,600 mg·kg-1) during the three weeks of CUMS significantly suppressed behavioral and biochemical changes induced by CUMS.As the sucrose preferences in 300,600 mg·kg-1 fingered citron volatile were 70.7%±10.2 % and 77.1%±11.9 %(P0.05 and P0.01);the horizontal scores in 150,300,600 mg·kg-1 fingered citron volatile were 87.7±15.4,98.3±19.7 and 110.3±28.9(P0.05,P0.05 and P0.01);the vertical scores in 300,600 mg·kg-1 fingered citron volatile were 24.5±6.3 and 29.2±7.2(P0.05 and P0.01);the serum corticosterone levels in 300,600 mg·kg-1 fingered citron volatile were(90.1±13.5) μg·L-1 and(77.5±10.7) μg·L-1(P0.05 and P0.01);the BDNF levels in 300,600 mg·kg-1 fingered citron volatile were 0.15±0.03 and 0.18±0.03(P0.05 and P0.01).Conclusion: The results suggest that the antidepressant-like actions of fingered citron volatile may be mediated by modulating the CORT concentration and increasing the expression of BDNF in hippocampus tissues.

Experimental Study on Antagonizing Liver Fibrosis of Macleaya cordata Extract

Abstract: Objective:To investigate the preventive and therapeutic effects of Macleaya cordata extract(MCE) on experimental liver fibrosis.Method:Experimental liver fibrosis models were induced by injection of carbon tetrachloride in combination with the drinking of 5% alcohol on rats and Schistosoma japomicum infection in mice.The effect of MCE on anti-hepatic fibrosis was evaluated by comparing the liver index,tissue biochemical indices(ALT,AST),lipid peroxidation indices(GSH-Px,MDA,SOD),serum fibrosis indices(HA,PCⅢ,LN),the expression levels of Hyp and collagen type Ⅰ,Ⅲ(CoⅠ,CoⅢ),the liver pathology before and after MCE intervention.Result:In CCl4-induced liver fibrosis model in rats,Compared with model group,the liver index,the level of AST,the expression of CoⅠwere obviously decreased in MCE preventive groups,and high-dose preventive MCE could significantly reduce the content of LN and the expressionof CoⅢ,middle-dose preventive MCE could significantly reduce the content of MDA and the expression of CoⅢ.The liver index,the contents of HA,MDA,the expression of CoⅢ were significantly decreased in MCE therapeutic group;in S.japomicum-induced liver fibrosis model in mice,preventive MCE could significantly reduce the content of ALT,the expression of Hyp and CoⅠ;the liver index,the contents of PCⅢ,LN,HA,AST,ALT in serum and the expression of CoⅠwere significantly decreased in high-dose MCE therapeutic group,and middle-dose therapeutic MCE could significantly reduce the levels of PCⅢ,HA,AST,ALT.In addition,the hepatic histopathology were also improved in varying degrees after MCE intervention.Conclusion:MCE has preventive and therapeutic effects on experimental liver fibrosis by inhibiting hepatic lipid peroxidation and alleviating the inflammation.

Research Advances of Jiegeng Tang

Abstract: The origin and pharmacological action of Jiegeng tang were reviewed and analyzied.The principle of composition,history,clinical practice and pharmacological action of Jiegeng tang were summarized and classified by consulting the CNKI database from 1992 to 2012.Jiegeng tang,the basic prescription for treating sore-throat and highly used in clinic,was originated from(Shanghan Lun).Modern pharmacological studies have shown that it was endowed with quite evident anti-inflammatory and expectorant effects.It is suggest that chemical composition analysis,material basis of therapeutic effects and proportion and mechanism of compatibility of Jiegeng tang should be proceeded in order to provide a basis of clinical application and further development.

Determination of the Effective Components in Different Parts and Harvest Time of Epimedium koreanum

Abstract: Objective:To determine the change regularity of the content of total flavones and icariin in different parts and harvest time of Epimedium koreanum to provide some new references for its optimized harvest time and further exploitation of its resource.Method: The content of flavonoids was determined by UV,and the content of icariin was determined by HPLC.Result: The content of flavonoids and icariin in E.koreanum is the highest in August,and it is the highest in the laminas and rhizomes,and the lowest in the stems and petioles.Conclusion: The laminas and rhizomes was of potential value in exploitation and utilization.It was suggested that August is the best time to harvest E.koreanum,and the stems and petioles should be discarded.

Effect of Environmental Ecological Factors on Saponins of Medicinal Plant

Abstract: To analysis the affect on the saponins of the medicinal plants bring by the major environmental factors.We analysis the relationship between the environmental factors and the composition of the saponins of the medicinal plants.the environmental factors,such as origin,harvest time,light,temperature,and cultivation measures etc.analysis the mechanism to find the theoretical basis for these phenomenons.The environmental factors such as origin,harvest season,growth years,light and temperature in the prosess of growth,and the regulate of the fertilizer or water in the prosess of cultivation,all of these have significant impact on the saponin of the medicinal plant.These results have certain theoretical support.The production of high quality of Chinese herbal medicines should consider the suitable area for the cultivation,and to determine the reasonable harvest time.The scientific cultivation practices in the cultivation is the effective means of to improve production and active ingredients of the medicinal plants.It is als a good way to take the appropriate short-term stress during the progress of optimize the cultivation conditions.

Effects of Danhong Injection on Protect Myocardium of Acute Coronary Syndrome Patients after Selective Percutaneous Coronary Artery Intervention

Abstract: Objective:Explore the effects of Danhong injection on protect myocardium of acute coronary syndrome(ACS) patients after selective percutaneous coronary artery intervention(PCI).Method: Using a prospective design of experiments,the 68 patients of the intended line of PCI with ACS were randomly divided into treatment group and control group,each group is 34 patients.At the same time into the hospital health examination in 40 cases as health group.Observation the level of all patients of plasma endothelin-1(ET-1),P-selectin(CD62p),high enzyme C reactive protein(hs-CRP) and after treatment of 7 d ST segment resolution rate at PCI preoperatively,immediately after operation,treatment of 14 d.Using SPSS 9.0 statistical software for data statistics and analysis.Result: ACS patients preoperative plasma levels of ET-1,CD62p,hs-CRP were higher than the healthy group,The differences were statistically significant(P0.05).The treatment group compared with the control group,the difference was not statistically significant 14 days after treatment,two groups of patients with ET-1,CD62p,hs-CRP levels were decreased compared with those before treatment,the difference was statistically significant(P0.05);The treatment group compared with the control group,treatment group,ET-1,CD62p,hs-CRP were lower than the control group,the difference was statistically significant(P0.05).After treatment of 7 d,the treatment group ST segment resolution higher than that in the control group,the difference was statistically significant(P0.05).Conclusion: Danhong injection can inhibit ASC in patients with PCI postoperative inflammatory reaction,protection of endothelial cells,is proposed as PCI postoperative myocardial protection measures in clinical treatment

Toxicity Study on Sophocarpine and Oxysophocarpine in Mice

Abstract: Objective:To explore the acute toxicity of sophocarpine and oxysophoca rpine in Kunming miceMethod:The drugs were administered in mice via the tail vein injectionThe toxic reactions were recorded and the medium lethal dose(LD50) was calculated by spss 130Result: The LD50 of sophocarpine and oxysophocarpine was 6394,25037 mg·kg-1,its 95% confidence interval was 5649-7308 mg·kg-1 and 21905-28802 mg·kg-1,respectivelyConclusion: Acute toxicity experiment showed that the sophocarpine and oxysophocarpine have some toxic effects on mice,and the former is more poisonous than the latter

GC-MS Analysis of Volatile Oil from Hedyotis lancea

Abstract: Objective:To study the chemical constituents of volatile oil from Hedyotis lanceaMethod: Two kinds of volatile oil were separately prepared by steam distillation(SD) and supercritical CO2 extraction(SFE)The constituents were separated and analyzed by gas chromatography-mass spectrometry with the area normalization method to determine the relative percentage content of each componentResult: Sixty-seven compounds were isolated and 32 compounds were identified by SD that composed about 7376% of the total essential oil;and 82 compounds were isolated and 45 compounds were identified by SFE that composed about 6162% of the total essential oilConclusion: The principal chemical constituents of the volatile oil from Hlancea are aliphatic derivatives,aromatic derivatives and terpene derivativesThere are some differences in the principal chemical constituents and content of the volatile oil extracted by SD and SFE