Open AccessJournal Article
The Frozen Cell
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This article is published in Journal of the Royal Society of Medicine.The article was published on 1970-11-01 and is currently open access. It has received 91 citations till now.read more
Citations
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Journal ArticleDOI
The Hofmeister effect and the behaviour of water at interfaces.
TL;DR: The first general, detailed qualitative molecular mechanism for the origins of ion-specific (Hofmeister) effects on the surface potential difference at an air-water interface is proposed; this mechanism suggests a simple model for the behaviour of water at all interfaces, regardless of whether the non-aqueous component is neutral or charged, polar or non-polar.
Journal ArticleDOI
A two-factor hypothesis of freezing injury: Evidence from Chinese hamster tissue-culture cells
TL;DR: In this paper, it was shown that cells frozen rapidly in 0.4 M solutions of sucrose, glycerol, and dimethyl sulfoxide are inactivated to a much greater extent by slow warming than are cells frozen slowly in those solutions; that is, cells frozen at rates greater than the optimum are considerably more sensitive to slow warming.
Journal ArticleDOI
Basic aspects of frozen storage of semen
TL;DR: The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane and modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are discussed.
Journal ArticleDOI
Factors affecting short-term and long-term stabilities of proteins
TL;DR: The current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses are discussed, and also the various factors to be considered for long-term storage of proteins in solution.
Journal ArticleDOI
Mechanisms of cryoinjury in living cells.
Doyong Gao,J. K. Critser +1 more
TL;DR: Factors and mechanisms of cryoinjury and its prevention are reviewed and discussed, including the most famous two-factor hypothesis theory of Peter Mazur, concepts of cold shock, vitrification, cryoprotective agens, lethal intracellular ice formation, osmotic injury during the addition/removal of CPAs and during the cooling/warming process, as well as modeling/methods in the cryobiological research.
References
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Journal ArticleDOI
The Hofmeister effect and the behaviour of water at interfaces.
TL;DR: The first general, detailed qualitative molecular mechanism for the origins of ion-specific (Hofmeister) effects on the surface potential difference at an air-water interface is proposed; this mechanism suggests a simple model for the behaviour of water at all interfaces, regardless of whether the non-aqueous component is neutral or charged, polar or non-polar.
Journal ArticleDOI
A two-factor hypothesis of freezing injury: Evidence from Chinese hamster tissue-culture cells
TL;DR: In this paper, it was shown that cells frozen rapidly in 0.4 M solutions of sucrose, glycerol, and dimethyl sulfoxide are inactivated to a much greater extent by slow warming than are cells frozen slowly in those solutions; that is, cells frozen at rates greater than the optimum are considerably more sensitive to slow warming.
Journal ArticleDOI
Basic aspects of frozen storage of semen
TL;DR: The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane and modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are discussed.
Journal ArticleDOI
Factors affecting short-term and long-term stabilities of proteins
TL;DR: The current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses are discussed, and also the various factors to be considered for long-term storage of proteins in solution.
Journal ArticleDOI
Mechanisms of cryoinjury in living cells.
Doyong Gao,J. K. Critser +1 more
TL;DR: Factors and mechanisms of cryoinjury and its prevention are reviewed and discussed, including the most famous two-factor hypothesis theory of Peter Mazur, concepts of cold shock, vitrification, cryoprotective agens, lethal intracellular ice formation, osmotic injury during the addition/removal of CPAs and during the cooling/warming process, as well as modeling/methods in the cryobiological research.