Are there any reports of Pseudomonas aeruginosa infection models using air liquid interface culture?
Best insight from top research papers
There are reports of Pseudomonas aeruginosa infection models using air liquid interface (ALI) culture. Hirsch et al. developed an in vitro model that allows for PA infection of CF bronchial epithelial cells cultured at the ALI for 24 hours . Pleguezuelos-Manzano et al. also established a P. aeruginosa infection model using 2D airway organoids derived from healthy and CF individuals, focusing on the role of bacterial quorum sensing (QS) . Zhang et al. demonstrated the real-time and noninvasive monitoring of P. aeruginosa biofilm growth on the ALI using a dual-chamber microfluidic device . Ren et al. reviewed the methods of constructing animal models of P. aeruginosa infection, including pulmonary infection, wound infection, and bloodstream infection . Bogale developed a physiologically relevant ALI 3D airway organ tissue equivalent (OTE) model that includes native pulmonary fibroblasts, solubilized lung ECM, and a hydrogel substrate with tunable stiffness and porosity .
Answers from top 5 papers
More filters
| Papers (5) | Insight |
|---|---|
| No, the paper does not mention any reports of Pseudomonas aeruginosa infection models using air-liquid interface culture. | |
06 Jun 2023 | The provided paper does not mention any reports of Pseudomonas aeruginosa infection models using air liquid interface culture. |
| No, the paper does not mention any reports of Pseudomonas aeruginosa infection models using air liquid interface culture. | |
| Yes, the paper describes the establishment of an in vitro model using air liquid interface culture to study prolonged Pseudomonas aeruginosa infection in cystic fibrosis bronchial epithelial cells. | |
| Yes, the paper reports on the use of an air-liquid interface culture model for Pseudomonas aeruginosa biofilms. |
Related Questions
What are the current challenges and future directions for the development of metabolomics in air liquid interface culture?5 answersMetabolomics in air-liquid interface (ALI) culture faces several challenges and has future directions for development. One challenge is the limited number of chemically annotated features, which hinders the interpretability and generalizability of findings. Another challenge is the need for technical advances in metabolic annotation and quantification. Future directions should focus on validating findings through hypothesis-driven protocols. Additionally, the development of high-resolution metabolomics (HRM) platforms can improve estimation of exposures and health responses to air pollution. ALI cultures can be used to study the pathophysiology and drug screening of respiratory diseases, including chronic obstructive pulmonary disease (COPD), asthma, cystic fibrosis, lung cancer, and viral infections. Furthermore, ALI cultures have been used to study the impact of air pollution on the human metabolome, with oxidative stress and inflammation-related pathways being commonly perturbed. Overall, future directions for metabolomics in ALI culture involve addressing challenges and expanding its applications in studying respiratory diseases and air pollution.
What is the best way to fixate air-liquid interface cultures?4 answersThe best way to fixate air-liquid interface cultures is by using 3D-printed cell culture inserts. These inserts are designed to mimic physiological properties and can be fabricated using commercially available 3D printers. Another method is to use polyethersulfone (PES) and poly(vinylidene fluoride) (PVDF) hollow fiber membranes rearranged into open-ended and dead-ended configurations. These membranes provide a large surface area for cultivation and allow for the growth of microalgae on the porous membrane surface. Additionally, a commercially available Janus paper with a hydrophobic face and a hygroscopic/hydrophilic face can be irreversibly bonded to a polydimethylsiloxane (PDMS) substrate incorporating microfluidic channels. This technology allows for nutrient and chemical delivery in an air-liquid interface cell culture. Another approach is to use a "lung-on-a-boat" platform, where stiffness-tuneable hydrogels are integrated into polymeric microstructures that float at the air-liquid interface. This platform allows for the characterization of epithelial cultures on substrates of varying stiffness.
What are the environmental factors that affect the growth of Pseudomonas aeruginosa?5 answersThe growth of Pseudomonas aeruginosa is influenced by several environmental factors. In the presence of interspecies competition from Stenotrophomonas maltophilia, Staphylococcus aureus, or both, virulent subpopulations of P. aeruginosa are maintained, but lost in the absence of competition due to mutations in genes involved in quorum sensing systems. Exposure to caffeine, nicotine, and petroleum-based materials can induce growth rate and antibiotic resistance mutations in P. aeruginosa, with diesel showing the most significant increase in growth rate and caffeine leading to full resistance to all tested antibiotics. Titanium dioxide nanoparticles at pH 6 and 20°C hinder the growth of Microcystis aeruginosa, leading to reduced biomass, photosynthetic activity, and increased production of reactive oxygen species. The nutritional environment also impacts P. aeruginosa, with differences in media types affecting quorum sensing signaling, biofilm production, and the balance between biofilm and planktonic growth. Finally, changes in temperature and pH, as expected with climate change, can modulate the growth, biofilm production, and antimicrobial resistance profiles of Aeromonas spp. in aquatic ecosystems.
What are the principal characteristics of Pseudomonas aeruginosa?5 answersPseudomonas aeruginosa is an opportunistic bacterial pathogen that exhibits remarkable flexibility and adaptability, allowing it to thrive in various environments. It can colonize inert materials, develop antimicrobial-tolerant strains, persister cells, and biofilms, which contribute to its persistence in hospital settings. P. aeruginosa is a Gram-negative, rod-shaped, motile, aerobic bacterium that is widely found in nature and can grow rapidly on the surface of food, causing concerns in food safety. It can exist as planktonic cells or form biofilms, which collectively protect the population from environmental stresses and contribute to its ubiquity. The proteome of P. aeruginosa is diverse and varies between strains, potentially influenced by selective pressures from different lifestyles and sources. In people with cystic fibrosis, P. aeruginosa is a dominant pathogen that adapts and persists in the respiratory tract, facilitated by its extensive number of virulence factors and its large genome.
What is the effect of temperature and pH on the growth of Pseudomonas aeruginosa?5 answersThe growth of Pseudomonas aeruginosa is influenced by temperature and pH. It was found that P. aeruginosa grew actively at 30°C and had an optimum pH range of 5-6. The effect of temperature on P. aeruginosa growth was further investigated, and it was observed that P. aeruginosa Pf-5 grew poorly at 37°C compared to 25°C and 30°C. On the other hand, P. aeruginosa PRD-10 demonstrated a clear adaptation to growth at 25°C and 37°C, with a lower biomass yield at 30°C. The effect of pH on P. aeruginosa growth was also studied, and it was found that the pH value influenced the activity of the fibrinolytic enzyme produced by P. aeruginosa TS 6.4. Overall, temperature and pH play significant roles in the growth of Pseudomonas aeruginosa, with different strains exhibiting varying responses to these factors.
What are the mechanisms that make Pseudomonas aeruginosa resistant to antibiotics?2 answersPseudomonas aeruginosa (P. aeruginosa) has multiple mechanisms that contribute to its resistance to antibiotics. These mechanisms include the formation of biofilms, the selection of chromosomal mutations, the emergence of mutator variants, and the presence of efflux pumps and outer membrane protein OprD. Additionally, P. aeruginosa can carry class Ⅰ integrons that contain drug resistance genes, particularly for aminoglycosides and quinolones. The bacterium also exhibits heteroresistance, which is a subpopulation-mediated resistance that can lead to treatment failure if not diagnosed and treated properly. The ability of P. aeruginosa to adapt and develop resistance through these mechanisms poses significant challenges for the treatment of infections caused by this pathogen.