Can i detect thymidine kinase negative cells by flow cytometry?
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Yes, thymidine kinase negative cells can be detected by flow cytometry. A new tool called microfluidic electroporative flow cytometry has been developed to detect the translocation of an EGFP-tagged tyrosine kinase, Syk, to the plasma membrane in B cells at the level of the cell population . This technique combines electroporation with flow cytometry and allows for the detection of protein translocation at the single-cell level. Additionally, intracellular flow cytometry using an enzymatic amplification approach called tyramide signal amplification (TSA) has been optimized for the assessment of intracellular kinase cascades . This approach improves the sensitivity and resolution of the assay, making it possible to measure proteins of low abundance and differentiate cells expressing subtly different protein concentrations. Therefore, flow cytometry can be used to detect thymidine kinase negative cells.
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| The provided paper does not specifically mention the detection of thymidine kinase negative cells by flow cytometry. | |
7 Citations | The paper does not mention the use of flow cytometry to detect thymidine kinase-negative cells. The paper is about the properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene and mechanisms of reversion to a thymidine kinase-negative phenotype. |
| The provided paper does not mention the detection of thymidine kinase negative cells by flow cytometry. | |
| The provided paper does not mention anything about detecting thymidine kinase negative cells by flow cytometry. |
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