Can metabarcoding be used to detect apicomplexan parasite diversity?
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Metabarcoding can indeed be utilized to detect apicomplexan parasite diversity. Studies have shown that next-generation high-throughput sequencing techniques, such as 18S rRNA amplicon sequencing, can effectively identify a wide range of apicomplexan parasites in various environments. This approach has been particularly successful in uncovering rare and unusual pathogens, like Trypanosoma evansi and Parabodo caudatus, in regions with limited information on vector-borne haemoparasites. Additionally, the use of multiple metabarcoding assays, along with a customized bioinformatic pipeline, has proven to enhance the detection probability of apicomplexan parasites while filtering out false positives. Therefore, metabarcoding emerges as a valuable tool for exploring the diversity of apicomplexan parasites and their potential host-parasite interactions in different ecosystems.
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| Papers (5) | Insight |
|---|---|
6 Citations | Yes, metabarcoding can detect apicomplexan parasite diversity in birds, including plasmodiid parasites, by using a 28S primer set with a custom blocking primer. |
| Yes, metabarcoding can detect apicomplexan parasite diversity, as shown in the study where it successfully identified Hepatozoon canis and Babesia vogeli infections in canines from the tropics. | |
| Yes, metabarcoding can detect apicomplexan parasite diversity, as shown in the study where eDNA metabarcoding revealed a positive correlation between Apicomplexa diversity and their invertebrate hosts in alpine habitats. | |
11 Apr 2023 | Yes, PacBio next-generation sequencing, a form of metabarcoding, effectively uncovers Apicomplexa diversity in various habitats, aiding in understanding parasite distribution and potentially influencing water quality standards. |
26 Aug 2020 3 Citations | Not addressed in the paper. |
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