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Does crosslinking of collagen type ! with EDC NHS cause osteogenic differentiation of MSCs? 

Tissue engineering
Nanofiber
Lysyl oxidase
Type I collagen
Bone regeneration
Best insight from top research papers

Crosslinking of collagen type I with EDC/NHS has been shown to impact osteogenic differentiation of mesenchymal stem cells (MSCs). Studies have demonstrated that collagen-based scaffolds, when crosslinked using different methods like genipin or EDC/NHS, can influence the adhesion, proliferation, and osteogenic differentiation of MSCs . While the mechanical properties of the scaffolds remain relatively stable post-crosslinking, changes in scaffold chemistry affect cellular behaviors such as adhesion and differentiation of MSCs . Inhibition of collagen crosslinking has been found to impair the osteogenic program in pre-osteoblast cells, indicating that collagen crosslinking plays an instructive role in differentiation processes . Therefore, crosslinking of collagen type I with EDC/NHS can indeed influence the osteogenic differentiation of MSCs, highlighting the importance of scaffold chemistry in tissue engineering applications.

Answers from top 5 papers

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Papers (5)Insight
Open access•Posted Content•DOI
Application of EGCG modified EDC/NHS cross-linked extracellular matrix to promote macrophage adhesion
Chenyu Chu, Shengan Rung, Renli Yang, Yi Man, Yili Qu 
04 Oct 2020-bioRxiv
2 Citations
Crosslinking collagen type I with EDC/NHS does not directly cause osteogenic differentiation of MSCs. However, it promotes cell adhesion, viability, and vascularization, enhancing bone regeneration potential.
Journal Article•DOI
Collagen functionalized bioactive nanofiber matrices for osteogenic differentiation of mesenchymal stem cells: bone tissue engineering.
Yixing Cheng, Daisy M. Ramos, Paul Lee, Danni Liang, Xiaojun Yu, Sangamesh G. Kumbar 
01 Feb 2014-Journal of Biomedical Nanotechnology
66 Citations
Yes, crosslinking collagen type I with EDC NHS promotes osteogenic differentiation of MSCs on nanofiber matrices, enhancing adhesion, proliferation, and expression of osteogenic markers like ALP and mineralization.
Journal Article•DOI
The Role of Collagen Crosslinking in Differentiation of Human Mesenchymal Stem Cells and MC3T3-E1 Cells
Hugo Fernandes, Koen J. Dechering, Eugene P. van Someren, Ilse Steeghs, Marion Apotheker, Anouk Leusink, Ruud A. Bank, Karolina Janeczek, Clemens van Blitterswijk, Jan de Boer 
03 Aug 2009-Tissue Engineering Part A
49 Citations
Not addressed in the paper.
Open access•Journal Article•DOI
Osteogenesis and mineralization of mesenchymal stem cells in collagen type I-based recombinant peptide scaffolds.
Kendell M. Pawelec, Davide Confalonieri, Franziska Ehlicke, Huibert A. van Boxtel, Heike Walles, Sebastiaan G. J. M. Kluijtmans 
01 Mar 2017-Journal of Biomedical Materials Research Part A
24 Citations
Crosslinking collagen type I with EDC NHS was not specifically studied in the paper. However, crosslinking with different methods did not hinder osteogenic differentiation of MSCs in recombinant peptide scaffolds.
Journal Article•DOI
Osteogenic differentiation of BMSCs in collagen-based 3D scaffolds
Tingting Zhang, Tingting Zhang, Hong Chen, Hong Chen, Yajie Zhang, Yue Zan, Yue Zan, Tianyu Ni, Min Liu, Renjun Pei, Renjun Pei 
21 Jan 2019-New Journal of Chemistry
1 Citations
Crosslinking collagen with EDC NHS in a scaffold with carboxymethyl chitosan and hyaluronic acid promotes osteogenic differentiation of BMSCs, enhancing adhesion, proliferation, and osteogenic markers secretion.

Related Questions

Is sucrose is used for crosslinking of collagen?4 answersSucrose is indeed used for crosslinking of collagen, as indicated in the research studies. One study compared the effects of different carbohydrates on scleral crosslinking and found that sucrose, along with ribose and glycogen, was used for sub-Tenon injections to induce collagen crosslinking in rabbit sclera. Another study focused on a sugar cross-linked resorbable collagen membrane, where sucrose was utilized as a crosslinker to create a barrier membrane that remained impermeable to cells and vessels over 30 days. Additionally, sucrose was oxidized to polar polyaldehydes to crosslink ultrafine fibrous scaffolds from corn protein, demonstrating its effectiveness in improving water stability without cytotoxicity in protein-based materials. These findings collectively highlight the role of sucrose in collagen crosslinking for various applications.
Is glucose is used for crosslinking of collagen?5 answersGlucose is not specifically mentioned as a crosslinking agent for collagen in the abstracts provided. However, the abstracts do mention the use of other sugars, such as ribose, oxidized oligosaccharides, and partially oxidized sugars like glucose, fructose, maltose, sucrose, and raffinose. These sugars are used as crosslinkers to enhance the structural integrity and stiffness of collagen matrices, and to form stable hydrogel microenvironments for 3D cell culture. The crosslinking reactions involve the introduction of aldehyde groups from the sugars, which then react with collagen molecules or aggregates to form crosslinked structures. The resulting crosslinked collagen materials exhibit improved mechanical properties, resistance to enzymatic digestion, and good biocompatibility. The use of sugars as crosslinkers offers advantages over traditional crosslinking agents like glutaraldehyde and formaldehyde, which can be cytotoxic.
How do we use EDC-NHS as crosslinker in natural gum and chitosan based hydrogels?5 answersEDC-NHS is used as a cross-linking agent in natural gum and chitosan-based hydrogels. Gelatin, a natural biopolymer derived from collagen, can be cross-linked using EDC-NHS to improve its mechanical properties. Similarly, a mixture of silk fibroin, collagen, and chitosan can be cross-linked using EDC/NHS to create three-dimensional scaffolds with improved physico-chemical properties. Chitosan oligosaccharide-graft-acrylic acid (CSO-g-AA) can also be cross-linked using EDC and NHS to prepare polyacrylamide (PAM) hydrogels. Additionally, EDC and NHS can be used as cross-linking agents in sodium alginate (SA)/chitosan (CS) hydrogels, where the addition of EDC/NHS leads to reinforced three-dimensional networks and improved mechanical properties. Overall, EDC-NHS is commonly used as a cross-linking agent in natural gum and chitosan-based hydrogels to enhance their properties and create stable network structures.
What is collagen cross link in infectious keratitis?4 answersCollagen cross-linking (CXL) is a treatment method for infectious keratitis that involves using UV rays and riboflavin to inactivate pathogens and strengthen the corneal stroma. CXL acts as a photo mediator to inactivate pathogens in plasma, platelets, and red blood cells, while also inducing a change in the properties of collagen and increasing the resistance of the corneal stroma to bacterial degradation. It has been shown to be effective in managing infectious keratitis and preventing the progression of corneal melting. Studies have reported positive outcomes with the use of CXL in infectious keratitis, although further evaluation and development of treatment protocols are needed.
What kind of collagen is best for osteoporosis?4 answers
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