Does mitomycin C induce interstrand DNA crosslinks?
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Yes, mitomycin C (MC) induces interstrand DNA crosslinks (ICLs). MC, along with its derivative decarbamoylmitomycin C (DMC), forms ICLs between deoxyguanosine residues on opposing DNA strands. These ICLs are crucial for the cytotoxic effects of mitomycins on cancer cells by inhibiting DNA replication. The formation of ICLs by MC and DMC is diastereospecific, with trans-ICLs predominantly at CpG sequences and cis-ICLs at GpC sequences. Different cellular responses are triggered by trans and cis-ICLs, with evidence suggesting that DMC-induced ICLs lead to p53-independent cell death, important for treating tumors with mutant p53. Proteomic studies have identified various molecular targets and cellular responses involved in DNA repair and cell cycle regulation upon treatment with these ICLs.
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| Papers (5) | Insight |
|---|---|
1 Citations | Yes, Mitomycin C (MC) induces interstrand crosslinks (ICLs) in DNA, along with monoadducts. The study focuses on synthesizing oligonucleotides containing MC-DNA adducts to investigate their biological effects. |
3 Citations | Yes, Mitomycin C (MC) induces interstrand DNA crosslinks between deoxyguanosine residues on opposing DNA strands, forming primarily trans-crosslinks at CpG sequences and cis-crosslinks at GpC sequences. |
| Yes, Mitomycin C (MC) induces interstrand DNA crosslinks, specifically trans-ICLs in MCF-7 cells. Decarbamoylmitomycin C (DMC) forms cis-ICLs preferentially. | |
1 Citations | Yes, Mitomycin C (MC) induces interstrand crosslinks (ICLs) by undergoing nucleophilic attack by DNA bases, contributing to its cytotoxic effects as an alkylating agent in cancer treatment. |
| Mitomycin C induces interstrand DNA crosslinks, forming trans-ICLs in 5’-CpG sequences. These crosslinks are cytotoxic and affect cellular responses in K562 cells. |
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