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How 3D bioprinting can be used to produce substrate for mushroom cultivation? 


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3D bioprinting can revolutionize substrate production for mushroom cultivation by utilizing innovative biomaterials like mycelium-based composites. These composites, derived from fungi mycelium grown on organic substrates like waste cardboard , can be shaped intricately through 3D printing, reducing mold waste and enabling the fabrication of complex forms. Additionally, Cold Atmospheric Plasma (CAP) treatment can be applied to substrates to effectively reduce bacteria growth, enhancing substrate quality for mushroom cultivation . Furthermore, incorporating litsea cubeba extract in substrate auxiliary materials can provide essential nutrients and protect mushrooms from diseases and pests . By integrating these techniques, 3D bioprinting can offer sustainable, efficient, and contaminant-free substrate solutions for optimizing mushroom cultivation processes.

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3D printing can create mycelium-based composites for mushroom cultivation by using waste cardboard as a substrate, reducing waste and enabling intricate forms with minimal mold usage.
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Related Questions

What are the steps needed to be followed in good compost making through mushroom cultivation?5 answersTo create high-quality compost for mushroom cultivation, several key steps must be followed. Firstly, prepare a nutrient-rich mixture containing materials like bagasse, sawdust, cob, bran, peat, edible mushroom polysaccharide, and bean pulp, along with a nutrient solution including calcium amino acid chelate, selenium amino acid chelate, urea, and other components. Next, sterilize and cool the mushroom cultivation raw material in a sterile environment after implantation, ensuring a clean and suitable growing medium. Then, mix sisal residues, cassava bark residues, dried cow dung, gypsum, calcium superphosphate, quick lime, and peanut bran to create compost with controlled water content, air humidity, and pH value. Finally, inoculate and culture the prepared mixture in a rod bag, managing the process carefully to enhance mushroom growth and yield.
How do different substrates affect the growth and yield of functional mushrooms?5 answersDifferent substrates have varying effects on the growth and yield of functional mushrooms. For example, in the study by Bayo et al., it was found that different substrates, such as saw dust, cotton waste, wheat straw, and corncob, had different yields and biological efficiencies for Pleurotus ostreatus mushrooms. Similarly, Suwannarach et al. highlighted that substrate composition and microbial diversity play a crucial role in mushroom production, affecting both the quality and quantity of yields. Additionally, Afify et al. found that mixed substrates with rice straw had a positive impact on spawn running, pinhead formation, and fruiting body formation for Pleurotus florida mushrooms. Furthermore, Edo and Shareef observed that different substrates, such as oat, corn, millet, wheat, and canary seed, influenced the growth and yield of Pleurotus ostreatus mushrooms, with variations in colonization time, pinhead formation, and number of heads per bag. Overall, the choice of substrate can significantly affect the growth and yield of functional mushrooms, highlighting the importance of substrate selection in mushroom cultivation.
How to grow mushrooms?4 answersA method of growing mushrooms involves using blocks of compost to form a composite compost bed. The blocks are laid out on the ground side-by-side to form an elongate composite bed. The top cover of each block is removed, and a layer of growing material is formed on top of the composite bed using a carriage with a container for growing material. The top surface of the growing material is then leveled to form a desired thickness. Another method for commercially growing mushrooms includes placing a layer of compost in a bed, laying a polymer sheet over it, and placing a layer of peat over the sheet. The mushrooms are grown in the peat layer, harvested, and then a replacement polymer sheet and layer of peat are placed for growing a replacement crop. There is also a method that involves building a cultivation room, selecting seeds, preparing materials, inoculating, cultivating hyphae, managing the mushroom growth period, and harvesting. Additionally, a method for cultivating edible mushrooms includes putting a culture medium into a bottle, sterilizing it, inoculating mushroom fungus, and forming mycelium by cultivating the fungus. Another method involves growing edible mushrooms by mixing material lumber or sawdust with a hypha extract and a culture medium, and then culturing the edible mushroom in a cultivation room.
Why Plant Cells for 3D bioprinting?3 answersPlant cells are being used for 3D bioprinting due to their unique properties and potential applications. One of the main advantages of using plant cells is their totipotency, which allows any part of the plant to regenerate a complete individual, making them ideal for tissue engineering and regenerative medicine. Additionally, plant cells have a high growth rate, making them a convenient tool for studying cellular reprogramming and cell cycle reentry. Furthermore, plant cells can be used to generate functional tissues and organs, offering possibilities for biomedical and other applications. Plant-Microbial Fuel Cells (PMFCs) are another area where plant cells are being utilized. PMFCs can generate clean electricity on-site without harming the plants, making them suitable for simultaneous agriculture and power generation. Overall, the use of plant cells in 3D bioprinting provides opportunities for studying cellular functions, tissue regeneration, and developing low-cost solutions for various applications.
What is the benefit of rice straw as substrate for mushroom?3 answersRice straw is a beneficial substrate for mushroom cultivation due to its positive effects on mushroom growth and yield. Studies have shown that using rice straw as a substrate, either alone or in combination with other materials, can result in significant improvements in physical crop traits such as cap diameter, cap weight, fruit body weight, and early yield. Additionally, the use of rice straw as a substrate enhances the proportions of dry matter, phosphorous, nitrogen, protein, and total carbohydrates in the fruit bodies. Furthermore, rice straw has been found to be an effective and economical substrate for oyster mushroom cultivation, with good mushroom yield performance and nutritional content. Overall, the use of rice straw as a substrate provides a sustainable and environmentally friendly method for mushroom cultivation, utilizing agricultural waste materials and reducing pollution.
Potential of saw dust as substrate for mushroom cultivation?1 answersSawdust has shown potential as a substrate for mushroom cultivation. Studies have demonstrated that supplementation with scallop shell powder (SSP) in sawdust substrates can improve the growth of king oyster mushrooms (P. eryngii). Another study developed a mushroom cultivation substrate using sawdust, corncob, corn straw powder, wheat bran, calcium carbonate, gypsum, and white sugar, which resulted in a high biological efficiency of 63.59%. Additionally, a culture medium consisting of sawdust, corncobs, bran, and gypsum was found to widen the range of raw material sources for mushroom cultivation, reduce production costs, and increase fruiting yield. Furthermore, softwood substrates, including sawdust, fermented with liquid digestate from a biogas station, showed successful growth of Pleurotus ostreatus, P. eryngii, and Ganoderma lucidum, indicating the potential for utilizing sawdust as a substrate for mushroom production. Finally, a sawdust medium containing oak sawdust, calcium carbonate, wheat bran, cotton seed hull, black sugar, water, nano detoxication sulphur, and anthocyan was found to activate starter cultures and promote mushroom growth.

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