How can the concentration of nanoparticles be determined by the UV Vis graph?
Best insight from top research papers
The concentration of nanoparticles can be determined using UV-Vis spectroscopy. This technique relies on the fact that nanoparticles scatter and absorb light, allowing them to be detected using a traditional UV-Vis spectrophotometer . By measuring the absorption of light at specific wavelengths, the concentration of nanoparticles can be determined, provided that a reference material or a well-understood material is available . However, UV-Vis spectrophotometry alone cannot simultaneously determine the concentration, size, or refractive index of nanoparticles . It is recommended to use UV-Vis spectrophotometry in combination with other methods for a more accurate determination of these parameters . In the case of metal nanoparticles, plasmonic features in the UV-Vis spectrum can provide information about local refractive index, particle size, shape, and agglomeration . However, it is not recommended to rely solely on UV-Vis spectrophotometry for measuring these parameters due to associated uncertainties .
Answers from top 3 papers
More filters
| Papers (3) | Insight |
|---|---|
| The paper states that the UV spectroscopy method is not suitable for determining the concentration of protein nanoparticles, as the reaction of the cross-linker with the protein changes the UV absorbance. Therefore, the paper does not provide information on how to determine the concentration of nanoparticles using the UV Vis graph. | |
| The concentration of nanoparticles can be determined using UV-Vis spectrophotometry if a reference material is available or if the material is well understood. | |
| The concentration of nanoparticles can be determined by integrating the absorbance concentration equation and the volume concentration equation, as stated in Equation (10) in the paper. |
Related Questions
How is nanoparticle concentration determined from UV-Vis absorbance?4 answersNanoparticle concentration can be determined from UV-Vis absorbance using various methods outlined in the provided research contexts. UV-Vis spectroscopy is commonly employed for this purpose, especially in the case of gold and silver nanoparticles. The Beer-Lambert law, Mie theory, and log-normal probability distribution of nanoparticle sizes are utilized for fitting UV-Vis spectra to determine concentration. Additionally, the extinction at 400 nm is a reliable method for in situ determination of gold nanoparticle concentration, even in the presence of interference factors. For silver nanoparticles, a new method based on IBT absorption in the UV region has been proposed, showing good agreement between experimental and theoretical values. Furthermore, a quantitative method for determining metal oxide nanoparticle concentration synthesized by laser ablation in liquid has been demonstrated, incorporating volume analysis and size distribution.
What does uv vis spectroscopy tells about propety of nanoparticles?5 answersUV-Vis spectroscopy provides valuable insights into the properties of nanoparticles. It is a widely used method for measuring the optical properties and electronic structures of nanoparticles, especially metal nanoparticles, which are crucial for various high-technology applications. This technique allows the determination of the concentration of particles, although it may not provide simultaneous information on size or refractive index. Additionally, UV-Vis spectroscopy can be utilized to calculate the optical band gap of materials, aiding in the selection of materials for energy conversion purposes like solar cells. Moreover, UV-Vis spectroscopy can be employed to quantify molecules bound to nanoparticles accurately, offering essential information for applications in drug delivery and biosensors.
Can uv-vis be used to determine the bandgap of ZnO nanoparticleas?5 answersUV-vis spectroscopy can be used to determine the bandgap of ZnO nanoparticles. The absorbance values of ZnO nanoparticles can be measured using a UV-Vis Spectrophotometer in the wavelength range of 275-875 nm. The band gap value of ZnO nanoparticles can be analyzed using UV-vis absorbance measurements and Kubelka-Munk theory. The energy band gap of ZnO nanocrystallites can be successfully tuned in the range of 3.15 - 3.02 eV depending on the nanocrystallite size. The UV-vis absorption spectrum of ZnO nanoparticles can be simulated using time-dependent density functional theory (TDDFT). The experimental nanoparticle's spectrum and the simulated UV-vis spectrum show good agreement. Therefore, UV-vis spectroscopy is a suitable technique for determining the bandgap of ZnO nanoparticles.
What is the concentration and size of nanofluid particle?5 answersThe concentration and size of nanofluid particles vary across the different papers. In the study by Yapici et al., the particle mass concentrations ranged from 5 to 20 wt %, and the particle sizes varied for each metal oxide nanoparticle. Ramya et al.investigated the thermal diffusivity of water-based ZnO nanofluid and found that the thermal diffusivity increased with increasing particle size and concentration. Ting et al.used SiO2 nanoparticles with sizes ranging from 20 to 30 nm, 60 to 70 nm, and 400 nm in their solar pond experiment. Minakov et al.studied the evaporation of nanofluids and found that the evaporation rate increased with an increase in nanoparticle concentration. Tengku Mohd et al.analyzed nanofluid viscosity and found that it increased with decreasing particle size and increasing volumetric concentration.
What are the different concentrations of nanoparticles that have been studied?5 answersDifferent concentrations of nanoparticles have been studied in the papers. One paper examined the concentrations of TiO2 and SiO2 nanoparticles, with 0.01wt% and 3wt% being used respectively. Another paper investigated the addition of Ni ions to nano-ferrite, with concentrations of NixCo1-xFe2O4 ranging from x=0.0 to x=1.0. A third paper focused on the acute toxicity of chitosan-coated silver nanoparticles, using doses of 50, 25, and 10 mg/kg body weight. The fourth paper explored the anti-hyperuricemic effectiveness of silver oxide nanoparticles, with doses of 5, 10, and 20 µg/mL being administered. Lastly, a fifth paper studied the properties and composition of natural nanoparticles, specifically kaolinite, montmorillonite, and muscovite, without specifying the concentrations.
How is logP determined in nanoparticles?3 answersLogP in nanoparticles is determined by modifying the lipophilicity of the drug and using bioadhesive characteristics for drug delivery. N-acyl-gemcitabine derivatives with different solubility and logP were synthesized and loaded into PLGA nanoparticles. The drug content increased with lipophilicity, but the subsequent release decreased. Lipophilicities of molecules can be determined using chromatography-based approaches and/or the COSMO-RS method. High logPo/w values, up to around 20, were obtained for complex hydrogen-bonding molecules. A quantitative structure-property relationship (QSPR) study was conducted to determine LogP. The best QSPR formula included three quantum mechanics descriptors and did not include molecular fragments' descriptors. LogP can also be determined using a flask shaking method and a two-phase system solvent. A novel approach to high-throughput logP measurement based on liquid chromatography/ultraviolet/mass spectrometry (LC/UV/MS) has been proposed.