How does bubble size affect plant cell culture in a bioreactor?
Best insight from top research papers
Bubble size has a significant impact on plant cell culture in a bioreactor. Different bubble sizes can affect the growth and productivity of plant cells. In a study by Sandesh et al., it was found that the volumetric productivity in a bubble column bioreactor was nearly 2.4 times higher compared to shake flask, and a high flow rate of air into the bubble column hindered cell growth by foaming . Another study by Yang et al. showed that the size of bubbles produced by aeration influenced the lipid production of microalgae. The system with a bubble size of 1.5 mm achieved the highest lipid productivity, while the system with a bubble size of 3.5 mm had the highest biomass productivity . These findings suggest that bubble size plays a crucial role in the growth and productivity of plant cells in a bioreactor.
Answers from top 4 papers
More filters
| Papers (4) | Insight |
|---|---|
17 Citations | The provided paper is about the effects of air bubble size on algal growth rate and lipid accumulation in photobioreactors. It does not provide information about how bubble size affects plant cell culture in a bioreactor. |
2 Citations | The provided paper is about the impact of bubble size on docosahexaenoic acid (DHA) production by Crypthecodinium cohnii in a bubble column bioreactor. It does not provide information about how bubble size affects plant cell culture in a bioreactor. |
01 Jan 2016 | The provided paper does not mention how bubble size affects plant cell culture in a bioreactor. |
17 Citations | The provided paper does not directly mention the effect of bubble size on plant cell culture in a bioreactor. |
Related Questions
What is a useful volume for a bioreactor for bioprinting?5 answersA useful volume for a bioreactor in bioprinting applications can vary depending on the specific requirements of the process. Different studies have demonstrated the effectiveness of bioreactors with varying volumes. For instance, a 1 mL polymer inkjet 3D printed microbioreactor was successfully used for optimizing recombinant protein production. On the other hand, a scalable dual perfusion bioreactor platform supporting up to 96 samples with two fluid circuits was developed for multi-organ-on-a-chip applications. Additionally, a bioreactor designed for tendon tissue engineering showcased improved mechanics and ECM content while withstanding continuous load for 3 weeks. Therefore, the ideal volume for a bioreactor in bioprinting depends on the specific application and goals of the research or production process.
Which physicochemical factors affect bubble generation in DAF?4 answersBubble generation in dissolved air flotation (DAF) is affected by several physicochemical factors. The salinity of the water has been found to impact bubble size, with smaller bubbles formed under increased salinity. Temperature also plays a role, as higher temperatures result in smaller bubble sizes. The operating pressure is another important factor, with bubble size decreasing up to a critical pressure of 3.5 atmospheres. The surface characteristics (zeta potential) of both bubbles and particles, as well as the sizes of the bubbles and particles, also influence bubble generation. Additionally, the addition of coagulants such as alum and ferric salts can affect bubble volume concentration (BVC) and bubble characteristics. Overall, these factors contribute to the optimization of the DAF process for efficient bubble generation and subsequent particle removal.
What gases are good for plant cell culture?5 answersPlant cell culture requires a gaseous environment that supports normal growth and development. The gases that are important for plant cell culture include carbon dioxide (CO2) and ethylene (C2H4). CO2 is essential for photosynthesis and respiration, while ethylene plays a role in regulating developmental patterns. In addition to these gases, air or gas mixtures of known composition can be used to continuously flush out the gaseous environment within the culture flasks. This helps to maintain the cultures and overcome problems such as contamination and desiccation of the medium. Overall, a controlled gaseous environment with appropriate levels of CO2 and ethylene, along with proper ventilation, is crucial for successful plant cell culture.
What is the bubble that the elodea produce during photosynthesis?5 answersDuring photosynthesis, Elodea canadensis produces bubbles that are a result of the process of CO2 fixation. These bubbles are formed as a byproduct of the photosynthetic O2 evolution and photosynthetic CO2 fixation. The observed increase in pH (ΔOH+) in the external solution is primarily caused by CO2 fixation. However, there is also a ΔOH+ independent of CO2 fixation, which is more closely linked to photosynthetic electron flow. The H+: O2 ratios observed during experiments suggest that CO2 fixation is the main cause of the apparent ΔOH+. Therefore, the bubbles produced by Elodea during photosynthesis are a result of the release of oxygen gas as a byproduct of CO2 fixation.
Why do small bubbles have a greater impact on mammalian cells than large bubbles at low aeration rates?5 answersSmall bubbles have a greater impact on mammalian cells than large bubbles at low aeration rates because the energy dissipation rates (EDR) are higher for smaller bubbles, leading to more cell damage. Even though the volume exceeding a particular EDR increases with bubble size, smaller bubbles have a more significant impact on a volume-to-volume basis. This is supported by numerical simulations and high-speed experiments. The damage caused by bubbles is influenced by their adhesion to cells, with floating bubbles causing more harm than adhered bubbles. Adhered bubbles can protect cells against floating bubbles, but excessive bubbles can still cause damage. Therefore, the size of the bubbles plays a crucial role in determining the impact on mammalian cells, with smaller bubbles having a greater potential for cell damage.
Why is better used bioreactor system to scale the protein production?5 answersUsing bioreactor systems for scaling protein production is advantageous for several reasons. Firstly, bioreactors allow for the precise modeling and scaling of bioreactor processes, aiding in the industrialization of laboratory processes. Secondly, bioreactors provide the ability to control growth conditions through real-time monitoring, facilitating further optimization of the protein production process. Additionally, bioreactors offer the potential for high-yielding production of recombinant proteins in yeast, allowing for more reproducible and efficient production. Furthermore, the use of bioreactors enables the growth of cells at high density, which is beneficial for achieving high levels of protein expression. Overall, the use of bioreactor systems for scaling protein production offers improved control, reproducibility, and efficiency, making it a better choice for industrial applications.