How does the use of 30-60% ethanol affect the yield and composition of tomato leaf extracts?5 answersThe use of 30-60% ethanol in tomato leaf extracts has shown significant effects on plant growth and nematode control. Ethanol extracts from Neem, croton, and cassava plants demonstrated varying impacts on tomato plants. Additionally, ethanol has been found to influence the phytochemical profile and antioxidant potential of tomato by-products, with 99.5% ethanol yielding extracts with high phenolic content and strong antioxidant properties. Moreover, the inhibitory properties of ethanol extracts from Vernonia amygdalina and Cola acuminata on fungal pathogens in tomato fruits were observed, with complete inhibition of pathogens at higher concentrations. Therefore, the use of 30-60% ethanol can affect the yield and composition of tomato leaf extracts by influencing plant growth, antioxidant properties, and pathogen inhibition.
How do different extraction methods affect the yield and composition of bioactive compounds in plant extracts?5 answersDifferent extraction methods significantly impact the yield and composition of bioactive compounds in plant extracts. Various techniques such as maceration, Soxhlet extraction, ultrasound-assisted extraction, and microwave-assisted extraction have been studied for their effects on phytochemical profiles and biological activities. For instance, ultrasound-assisted extraction has shown high yields of bioactive compounds and strong antioxidant activity in green coffee bean extracts. Similarly, microwave-assisted water extraction has been found to be effective in isolating volatile compounds from Veronica taxa, with hydrosol extracts proving to be valuable sources of compounds for potential applications. The choice of solvent in ultrasound-assisted extraction has been crucial, with ethanol identified as the best solvent for recovering bioactive compounds from ora-pro-nobis leaves. Overall, the selection of extraction method and parameters plays a vital role in determining the quantity and quality of bioactive compounds obtained from plant materials.
What are the methods for leaf extraction to isolate the bioactive compounds?4 answersVarious methods are used for leaf extraction to isolate bioactive compounds from plants. Traditional methods such as maceration, percolation, and Soxhlet extraction are commonly employed. However, novel techniques have emerged to enhance efficiency and selectivity, including microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), pressurized liquid extraction (PLE), supercritical fluid extraction (SFE), and enzyme-assisted extraction. These modern approaches offer improved yields and efficiency in extracting bioactive compounds from plant materials. Ultrasound-assisted extraction (UAE) in particular has been found to be beneficial, as it requires less time and energy, operates at a lower temperature, and retains the quality of the extract. Additionally, innovative technologies such as high hydrostatic pressure (HHP), pulsed electric field (PEF), and supercritical fluid (SF) extraction are increasingly replacing conventional methods, resulting in higher extraction rates and lower impurities in the final extract.
What percent of ethanol is most used for plant extraction?4 answersThe percent of ethanol most commonly used for plant extraction varies depending on the study. In one study, the highest extraction yield of 34.52% was achieved using ethanol as the solvent. Another study found that the best factor setting for maximum extraction yields was 53% ethanol for stems, 45% for leaves, and 50% for bracts. Additionally, a study on Thunbergia laurifolia leaves found that the highest crude extract yield was obtained using 25% ethanol solvent. Therefore, the percent of ethanol used for plant extraction can range from 25% to 53%, depending on the specific plant material and extraction method employed.
How ph influencing extraction of bioactive compounds from fruits?5 answersThe pH of the extraction process can influence the extraction of bioactive compounds from fruits. In the case of prickly pear fruit, pulsed electric field (PEF) treatment resulted in increased juice yield and extraction of bioactive compounds, such as betalains, with higher total polyphenol content and antioxidant capacity. For blueberry fruits, moderate electric field (MEF) pre-treatment under different combinations of electric field strengths and heating temperatures increased the juice yield and the extraction of bioactive compounds, including total phenolics, total anthocyanins, and antioxidant activity. Pulsed electric field (PEF) pre-treatment of blueberry fruits also improved the extraction yield and antioxidant properties of the juice, as well as the recovery of bioactive compounds from the press cake. Overall, the pH of the extraction process plays a role in the efficiency of extracting bioactive compounds from fruits, and different techniques, such as PEF and MEF, can be used to optimize the extraction process.
How plant phytochemicals are obtained using Aqueous extraction?4 answersPlant phytochemicals can be obtained using aqueous extraction methods. One approach involves treating the plant matter with acidic components, such as amla fruits or lemon peels, which convert insoluble constituents into soluble forms, enabling the extraction of a wider range of constituents. Another method involves using subcritical water, where the temperature and pH of the water can be controlled during the extraction process. This allows for the extraction of different classes of phytochemicals from the plant material, which are separated into eluant subfractions corresponding to the temperature changes. Additionally, a process involving the use of a liquid polar solvent and a substantially immiscible nonpolar solvent can be employed. This process effectively extracts lignins, polar molecules, and tropolones into the polar solvent, resulting in a polar plant extract.