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How streptomycin? 


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Streptomycin is a compound that is used for various purposes. It can be used as a detection method for streptomycin itself, as described in the methods proposed by Jiang Weiyan et al. and Yang Min et al. . These methods involve different steps such as sample acidification, filtration, and purification to remove impurities and obtain a refined solution for detection. Streptomycin can also be used in the field of biology, specifically in the cultivation of large leaf tea trees, as described by Chen Huahong et al. . Additionally, streptomycin has been studied for its interaction with the ribosome and its role in tRNA discrimination, as shown in the research conducted by Catherine Spickler et al. and Kirill B. Gromadski et al. . These studies provide insights into the binding of streptomycin to the ribosome and its effect on the fidelity of aminoacyl-tRNA selection.

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Streptomycin is obtained through screening and culturing Streptomyces sp.E-3-R-1, which promotes the growth and disease resistance of large leaf tea trees.
Streptomycin interferes with the conformational coupling between codon recognition and GTPase activation on the ribosome.
Streptomycin binds to the decoding center of 16 S ribosomal RNA.

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What is the molecular mechanism of action of streptomycin?4 answersThe molecular mechanism of action of streptomycin involves multiple pathways. Streptomycin induces mitochondrial-dependent ferroptosis in non-adherent tumor initiating cells (TIC) from colon and breast cancer cell lines, leading to alterations in mitochondrial morphology and function. In bacterial cells, streptomycin has been shown to alter the activity of the mechanosensitive ion channel MscL, causing potassium efflux and potentially facilitating cell entry. Additionally, streptomycin's entry into bacterial cells may involve interaction with MscL, modifying its conformation to allow passage of dihydrostreptomycin into the cell. These findings highlight the diverse effects of streptomycin on both cancer cells and bacteria, shedding light on its intricate mechanisms of action.
What are the mechanisms by which Streptomyces produces melanin?5 answersStreptomyces produces melanin through various mechanisms. One mechanism involves the use of L-tyrosine as a precursor for melanin biosynthesis, which is activated by the transcriptional regulator AdpA. This activation leads to the production of tyrosinases and tyrosine peroxygenases, such as MelC2, MelD2, MelE, and LmbB2, which are involved in the conversion of L-tyrosine to melanin. Additionally, the production of melanin in Streptomyces can be visualized during conjugation, where a conjugative plasmid carrying the melC operon encoding an extracellular tyrosinase is transferred to a recipient strain, resulting in the synthesis of tyrosinase and subsequent melanin formation. These mechanisms highlight the role of L-tyrosine metabolism and the regulation of melanin biosynthetic genes in Streptomyces for melanin production.
How strB?5 answersThe strB gene has been identified in multiple studies. It has been found to be associated with streptomycin resistance in Escherichia coli strains, where it is located downstream of the strA gene. The strB gene encodes a hydrolase enzyme that can catalytically degrade pesticides containing an ester bond, such as deltamethrin and lambda-cyhalothrin. In Erwinia amylovora, the strA-strB gene pair is responsible for high-level streptomycin resistance, with strA encoding aminoglycoside-3"-phosphotransferase and strB encoding aminoglycoside-6-phosphotransferase. The hydrolase gene strB can also be used for removing pesticide residues in agricultural products and for constructing transgenic crops to produce pollution-free green agricultural products. Overall, the strB gene plays a crucial role in antibiotic resistance and pesticide degradation, offering potential applications in agriculture and environmental remediation.
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