How to determine the relative stoichiometry of protein to RNA in the particle?
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To determine the relative stoichiometry of protein to RNA in a particle, various methods can be employed based on the specific system under study. For instance, in the context of viral assembly, the stoichiometry of components like DNA-packaging pRNA and other structural proteins can be quantified using dilution factors, percentage probabilities, and titration curve slopes . In the case of negative-strand RNA viruses like Bunyamwera virus, the association of the N protein with RNA to form ribonucleoprotein complexes can provide insights into the stoichiometry, with approximately 12 nucleotides per N monomer being observed . Additionally, mass spectrometry-based techniques can be utilized to determine protein stoichiometries within complexes, as demonstrated in the study of spliceosomal complexes .
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Open access•Dissertation 07 Sep 2010 2 Citations | Relative stoichiometry of proteins to RNA in particles can be determined using mass spectrometry-based techniques like iTRAQ labeling and SILAC, as demonstrated in the study on spliceosomal complexes. |
28 Citations | The relative stoichiometry of protein to RNA in the particle can be determined by extracting and sequencing RNAs bound to the model RNPs, revealing approximately 12 nucleotides per N monomer. |
20 Feb 2022 | Relative stoichiometry of proteins in protein-RNA assemblies can be determined using mass spectrometry-based techniques like iTRAQ labeling, SILAC, and semi-quantitative spectral count analysis, as demonstrated in the study. |
| The relative stoichiometry of protein to RNA in the particle is determined by a simple biochemical model with resource competition using programmable and modular promoters in mammalian cells. | |
65 Citations | By comparing slopes of dilution factor curves, the stoichiometry of components can be determined. A slope of 1 indicates one copy per virion, while >1 suggests multiple copies. |
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