How to extract dna from serum samples using trizol method?
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To extract DNA from serum samples using the Trizol method, the following steps can be followed: Firstly, isolate total RNA, including miRNAs, from the serum using the Trizol-based method, ensuring efficiency and stability of miRNAs in stored conditions . Next, modify the traditional Trizol-based protocol to improve the extraction efficiency for serum microRNAs, making it suitable for real-time PCR-based profiling experiments . Additionally, consider using the TRIzol™ method for RNA and DNA co-extraction, which has been shown to be fast, simple, and economical, yielding higher DNA amounts compared to the phenol-chloroform method . Lastly, utilize the TRIzol-based frozen precipitation method (TFP method) for extracting protein functional effector sncRNAs (pfeRNAs) of approximately 30–60 nucleotides from plasma samples with high yield and quality .
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3 Citations | The optimized TRIzol-based method, as described in the study, can be used to efficiently extract circulating microRNAs from serum samples for diagnostic biomarker development. |
| DNA extraction from serum using Trizol involves isolating total RNA, including miRNAs, with Trizol®LS reagent, followed by quantification using qRT-PCR for gene expression analysis. | |
| Not addressed in the paper. | |
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