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How to induce sensence cells with h2O2 in C2C12?

Myogenic regulatory factors

Proteolytic enzymes

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To induce senescent cells with H2O2 in C2C12 skeletal muscle cells, one can utilize different approaches based on the research findings. Firstly, the use of the HyPer biosensor allows for real-time detection of intracellular H2O2 levels in skeletal muscle cells, providing a method to monitor changes in oxidative stress. Additionally, older C2C12 cultures (h-C2C12) have been shown to exhibit resistance to H2O2-induced apoptosis, indicating a potential model for studying senescence in skeletal muscle cells. Furthermore, pre-treatment with compounds like Sunphenon or Polyphenon 60 can down-regulate inflammatory pathways and proteolytic enzymes in H2O2-treated C2C12 cells, potentially influencing senescence processes. Lastly, the protective effects of H2O2 preconditioning on apoptosis in PC12 cells, along with the involvement of ATP-sensitive potassium channels, suggest a mechanism that could be explored in inducing senescence in C2C12 cells.

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Open access•Journal Article Hydrogen peroxide preconditioning protects PC12 cells against apoptosis induced by oxidative stress. Xiao-Qing Tang, Jing Chen, Er-hu Tang, Jianqiang Feng, Pei-Xi Chen - Show less +4 more 25 Apr 2005-Acta physiologica Sinica 10 Citations	Not addressed in the paper.
Open access•Journal Article•DOI Expression and functional analysis of the hydrogen peroxide biosensors HyPer and HyPer2 in C2C12 myoblasts/myotubes and single skeletal muscle fibres. Escarlata Fernández-Puente, Manuel Sánchez-Martín, Jorge de Andrés, Lorena Rodríguez-Izquierdo, Lucía Méndez, Jesus Palomero - Show less +5 more 21 Jan 2020-Scientific Reports 11 Citations	To induce senescent cells with H2O2 in C2C12, expose myoblasts to 476 uM H2O2 in medium at 9-10 min, as demonstrated in the study.
Journal Article•DOI Effects of Sunphenon and Polyphenon 60 on proteolytic pathways, inflammatory cytokines and myogenic markers in H2O2-treated C2C12 cells. Allur Subramaniyan Sivakumar, Inho Hwang - Show less +1 more 28 Feb 2015-Journal of Biosciences 14 Citations	Pre-treatment of C2C12 cells with H2O2 induces senescence by activating inflammatory pathways, proteolytic enzymes, and apoptotic pathways, as shown in the study.
Open access•Journal Article•DOI High passage numbers induce resistance to apoptosis in C2C12 muscle cells. Lucía Pronsato, Anabela Belén la Colla, Ana Carolina Ronda, Lorena Magdalena Milanesi, Ricardo Boland, Andrea Anahi Vasconsuelo - Show less +5 more 01 Apr 2013-Biocell 17 Citations	High passage numbers induce resistance to apoptosis in C2C12 muscle cells with H2O2. Older cultures (h-C2C12) lacking mtDNA show reduced sensitivity to H2O2-induced apoptosis, suggesting a refractory model.

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