How to measure and evaluate binding affinities?
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Binding affinities can be measured and evaluated by performing high-quality equilibrium binding measurements and documenting essential controls for incubation time and concentration regime . These controls are necessary to determine the reliability of the measurements and to avoid incorrect interpretations of affinities. A framework has been provided to guide researchers in evaluating, teaching, performing, and documenting binding measurements . Additionally, chemical shift perturbation titrations can be used to analyze binding data and determine the best method for fitting the data, such as simultaneous fitting of all raw shift data to a single dissociation constant . Residue-specific affinities can also be obtained from chemical shift changes of individual residues, with residues in close contact with the ligand having significantly stronger affinities . Overall, measuring and evaluating binding affinities involves considering parameters such as dissociation constants and using techniques like equilibrium binding measurements and chemical shift perturbation titrations .
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| Binding affinities can be measured and evaluated using techniques such as chemical shift perturbation titrations and statistical analysis methods like singular value decomposition. | |
| The binding reaction between an affinity substance and a binding partner is measured based on agglutination reactions, and evaluation is achieved by counting the level of agglutinated carrier particles. | |
197 Citations | The paper provides a framework for researchers to evaluate, teach about, perform, and document high-quality equilibrium binding measurements. |
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