How to reconstitute TGF-β1?
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To reconstitute TGF-β1, a scalable purification process from virally inactivated human platelets can be employed, where platelet lysates are treated with solvent/detergent, clarified, and chromatographed on an anion-exchange column to obtain a fraction enriched in TGF-β1 . Additionally, a complex involving a TGF-β1 inhibitory peptide and cyclodextrin has been developed for therapeutic purposes . The interaction between TGF-β1 and its receptors, T beta R-I and T beta R-II, has been extensively studied, showing that T beta R-II binds TGF-β1, leading to the recruitment and phosphorylation of T beta R-I, essential for downstream signaling . Furthermore, for abdominal aortic aneurysms, TGF-β1 has been encapsulated in PLGA nanoparticles to induce elastogenesis, demonstrating sustained release and bio-functionality of the protein .
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| Papers (5) | Insight |
|---|---|
| Reconstitute TGF-β1 by activating lyophilized TGF-β1 with acidic buffer containing BSA, followed by reconstitution in PBS with BSA before use, as detailed in the study. | |
85 Citations | TGF-β1 can be reconstituted by forming a complex between receptors TβR-I and TβR-II, where TβR-II phosphorylates TβR-I, initiating signal propagation to downstream substrates. |
14 Citations | TGF-β1 can be reconstituted by purifying it from virally inactivated human platelet lysates using a scalable chromatographic process, yielding a fraction enriched in TGF-β1 for potential therapeutic applications. |
| Not addressed in the paper. | |
| Not addressed in the paper. |
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