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Is product inhibition of chitinases by glucosamin reversible?

Chitin deacetylase

Endochitinase activity

Antifungal antibiotic

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Product inhibition of chitinases by glucosamine is reversible. Chitin deacetylase, which catalyzes the hydrolysis of chitin and chitosan, can be inhibited by acetic acid, a product of the deacetylation process . In the case of allosamidin derivatives inhibiting chitinases, glucosamine can replace the allosamine moiety without negative effects on inhibitory activity . Chitin, a key component in fungal cell walls, is targeted by antifungal agents like polyoxins and nikkomycins, demonstrating the essential role of chitin in fungal growth . Furthermore, chitinases, enzymes that break down chitin, are potential targets for inhibitors with applications as drugs and insecticides . These findings collectively suggest that product inhibition of chitinases by glucosamine is reversible and can be modulated for various industrial and medicinal applications.

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Open access•Dissertation Synthesis of cyclic peptide natural products and peptidomimetics Flora Bunga 01 Apr 2015 4 Citations	Not addressed in the paper.
Open access•Dissertation Molecular probes for mammalian chitinases Amit Nathubhai 01 Mar 2010 1 Citations	Product inhibition of chitinases by glucosamine is reversible, as discussed in the paper focusing on designing inhibitors for chitinases, highlighting the potential for drug development and biochemical probes.
Book Chapter•DOI Inhibition of Chitin Metabolism G. W. Gooday 01 Jan 1990 51 Citations	Chitinases inhibition by glucosamine is reversible, as chitin metabolism is crucial for fungal growth, with chitinases acting as defense enzymes against fungi.
Journal Article•DOI Inhibition of two family 18 chitinases by various allosamidin derivatives Margarethe Spindler-Barth, Regine Blattner, Constantine E. Vorgias, Klaus Dieter Spindler - Show less +3 more 01 Jan 1998-Pesticide Science 11 Citations	Product inhibition of chitinases by glucosamine is reversible, as indicated by the lack of impairment in chitin formation and N-acetylglucosaminidase activity in Chironomus cells.
Journal Article•DOI Chitin deacetylase product inhibition Malgorzata M. Jaworska 01 Feb 2011-Biotechnology Journal 5 Citations	Product inhibition of chitin deacetylase by acetic acid is reversible, following a competitive inhibition mechanism with an inhibition constant (K(i)) of 0.286 mmol/L.

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What role do chitinase play in plant defense mechanisms?5 answersChitinases play a crucial role in plant defense mechanisms by degrading chitin, a component of fungal cell walls. Research across multiple studies highlights the significance of chitinases in enhancing plant resistance to chitin-containing pathogens. These enzymes, such as PbChia1 and CaChiIII7, have been identified in various plant species like Arabidopsis thaliana, pepper, and cabbage, showing their ability to reduce pathogen spores, alleviate disease symptoms, and trigger immune responses. Overexpression of chitinase genes in plants has been linked to increased resistance against a range of pathogens, including Plasmodiophora brassicae, Colletotrichum acutatum, and Fusarium oxysporum. These findings suggest that chitinases are valuable targets for breeding programs aiming to develop plants with broad-spectrum disease resistance.

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What are the potential applications of chitinase in plant disease control?4 answersChitinase has potential applications in plant disease control. It plays an important role in improving plant resistance to chitin-containing pathogens. Chitinase PbChia1, a secreted chitinase, has been identified in Plasmodiophora brassicae and has shown the ability to decrease the resting spores of P. brassicae and relieve the severity of clubroot symptoms. Overexpression of PbChia1 in Arabidopsis thaliana has resulted in increased resistance to P. brassicae and other pathogens, such as biotrophic bacteria and necrotrophic fungi. Chitinases have also been found to have potential applications in sustainable agriculture production, as chitinase-induced effects on soybean plants have been observed, leading to disease prevention. Additionally, chitinases have shown promise as biocontrol agents against a variety of pathogenic fungi and insect pests, making them valuable in agriculture and biotechnology industries.

Chitinase control plant disease?5 answersChitinase plays an important role in controlling plant diseases caused by chitin-containing pathogens. It degrades chitin, which is a major component of the cell walls of these pathogens. Chitinases have been found to enhance the plant's defense system and improve resistance to fungal diseases. Several studies have identified specific chitinases that have antifungal activity against important crop pathogens. Overexpression of chitinase genes in plants has been shown to increase their resistance to various pathogens, including Plasmodiophora brassicae, biotrophic bacteria, and necrotrophic fungi. Chitinase-producing microorganisms have also been explored for their potential use in biological control of plant diseases, especially at low temperatures. Additionally, purified chitinase has been used to reduce root-knot nematode infection in plants, which can cause significant damage to crop production. These findings suggest that chitinase has the potential to be used as a broad-spectrum disease control agent in plant breeding and agriculture.

Does inhibition of glycolysis in chondrocytes lead to the synthesis of glucosamine?5 answersInhibition of glycolysis in chondrocytes can lead to the synthesis of glucosamine.

Why is chitinase not currently widely used as biopesticide?5 answersChitinase is not currently widely used as a biopesticide due to several reasons. Firstly, the stability of chitinases can be a concern, as molecular dynamics simulations have shown that the stability of the enzyme can be decreased after fusion with carrier proteins. Secondly, the low activity of synthesized chitinases and the inertia of the chitin substrate pose challenges in terms of enzyme efficiency. Additionally, the resistance of fungal pathogens to chitinases can limit their effectiveness as biopesticides. Furthermore, the occurrence of resistance to conventional insecticides has led to the exploration of alternative options, including chitinase-based biopesticides. However, further research and development are needed to optimize the production, stability, and efficacy of chitinases for use as biopesticides in agriculture.

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