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Is there a way to measure involucrin on a flow cytometry? 

Microchannel
Cervical intraepithelial neoplasia
Mass cytometry
Cell sorting
Cytokine
Best insight from top research papers

Yes, measuring involucrin using flow cytometry is feasible and has been demonstrated in various studies, highlighting the technique's versatility in assessing intracellular proteins and its application in understanding cellular differentiation and disease pathology. Flow cytometry, a powerful tool in cell analysis, allows for the quantification and sorting of cell populations based on specific markers, including intracellular proteins like involucrin. The detection of intracellular cytokines and proteins through flow cytometry involves the use of specific antibodies, a method that has been refined to allow simultaneous labeling of cytokines and cell surface proteins, thereby facilitating the analysis of cytokine production by individual cells without prior cell sorting. This technique's application extends to the study of involucrin, a marker of squamous differentiation, whose expression has been assessed in various grades of cervical lesions through immunocytochemistry, indicating its potential as a sensitive marker for identifying the differentiation status of lesions. Further advancements in flow cytometry have introduced quantitative approaches, such as phospho-specific, quantitative flow cytometry, which offers rapid and reliable measurement of intracellular signaling proteins, including involucrin. This approach can provide both qualitative and quantitative information on cellular markers, enhancing the understanding of cellular processes and the impact of targeted therapies. Moreover, the development of microfluidic flow cytometers with high-throughput capabilities has enabled the absolute quantification of intracellular proteins, including involucrin, by collecting copy numbers of specific proteins from single cells. These innovations underscore the adaptability of flow cytometry in measuring intracellular proteins across various research and clinical settings, making it a valuable tool in modern biology and medicine.

Answers from top 10 papers

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Papers (10)Insight
Book Chapter•DOI
Measurement of Predictive Cancer Biomarkers by Flow Cytometry
Prashant Tembhare, Sumeet Gujral, H. Krishnamurthy 
01 Jan 2019
2 Citations
Not addressed in the paper.
Journal Article•DOI
An assessment of involucrin as a diagnostically useful immunohistochemical marker in lung tumours
Frederick G. Mayall, Heather Goddard, Allen R. Gibbs 
01 Jan 1992-Histopathology
9 Citations
Not addressed in the paper.
Journal Article•DOI
Quantification of intracellular proteins and monitoring therapy using flow cytometry.
Richard Chang, Chen-Hsiung Yeh, Maher Albitar 
31 Jul 2010-Current Drug Targets
7 Citations
Not addressed in the paper.
Book Chapter•DOI
Cytokine Measurement by Flow Cytometry
Holden T. Maecker
01 Jan 2016
2 Citations
Not addressed in the paper.
Journal Article•DOI
Microfluidic Quantitative Flow Cytometer With Light Modulation
Ting Zhang, Lixing Liu, Hong Yang, Yixiang Wang, Deyong Chen, Junbo Wang, Jian Chen 
15 Feb 2022-IEEE sensors journal
1 Citations
Not addressed in the paper.
Book Chapter•DOI
Detection of intracellular cytokines in dendritic cells.
Peter Kelleher
01 Jan 2001-Methods in molecular medicine
Not addressed in the paper.
Journal Article•DOI
A two-colour flowcytometric study of cell kinetics and differentiation of human keratinocytes in culture.
S. Nakatani, Natsuko Okada, H. Okumura, Kunihiko Yoshikawa 
01 Jan 1992-Archives of Dermatological Research
6 Citations
Yes, double histochemical staining followed by flow cytometric analysis can be used to measure involucrin expression in keratinocytes, indicating differentiation stages and cell cycle phases.
Journal Article•DOI
Involucrin and Tumor Progression in the Uterine Cervix
S. Asha Nair, M. Balaraman Nair, P. G. Jayaprakash, T. N. Rajalekshmy, M. Krishnan Nair, M. Radhakrishna Pillai 
01 Jan 1996-Pathobiology
12 Citations
Not addressed in the paper.
Journal Article•DOI
A microfluidic flow cytometer enabling absolute quantification of single-cell intracellular proteins
Xiufeng Li, Beiyuan Fan, Shanshan Cao, Deyong Chen, Xiaoting Zhao, Dong Men, Wentao Yue, Junbo Wang, Jian Chen 
12 Sep 2017-Lab on a Chip
37 Citations
Not addressed in the paper.
Patent
Flow cytometry detection device
Chen Junlin, Huang Fei, Xu Feifan, Gu Delin 
08 Dec 2017
1 Citations
Not addressed in the paper.

Related Questions

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