Is there any bromelain extraction?
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Bromelain extraction methods have been studied in several papers. One study focused on comparing bromelain extracts from pineapple core and pulp, finding no significant differences in enzymatic activity and proteolytic activity between the two sources . Another paper discussed the various methods used for the extraction and purification of bromelain, including aqueous two-phase extraction, reverse micellar extraction, ultrafiltration, precipitation, and chromatographic techniques . A different study explored the extraction of bromelain from pineapple core waste and the stabilization of the enzyme as insoluble cross-linked aggregates, which showed improved thermal and pH stability compared to free bromelain . Another paper presented a method for extracting bromelain using pretreatment, enzymatic hydrolysis, isoelectric precipitation, ultrafiltration, and drying and preservation steps, with the resulting bromelain meeting pharmaceutical standards . Finally, ultrasonic-assisted extraction and microwave-assisted extraction were used to extract bioactive peptides, including bromelain, from pineapple byproducts, with the optimized extraction conditions resulting in high proteolytic activity .
Answers from top 5 papers
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| Papers (5) | Insight |
|---|---|
| Yes, bromelain extraction was conducted using ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) techniques in this study. | |
| Yes, the paper describes a method for extracting bromelain from pineapples or pineapple stems using a series of steps including pretreatment, enzymatic hydrolysis, isoelectric precipitation, ultrafiltration, and drying and preservation. | |
| Yes, the paper discusses various methods of bromelain extraction, including aqueous two-phase extraction, reverse micellar extraction, ultrafiltration, precipitation, and chromatographic techniques. | |
| Yes, the paper describes the extraction of bromelain from pineapple core waste using ammonium sulphate precipitation followed by cross-linking with glutaraldehyde. | |
| Yes, the paper discusses the extraction of bromelain from pineapple core as a strategy for pineapple waste valorization. |
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Supercritical fluid extraction (SFE) is another advanced technique, praised for its no-waste production, shorter extraction times, automation, and lower solvent consumption. SFE is particularly efficient in extracting pure antimicrobial pigments, showcasing high potential for application in various industries due to the biological and antimicrobial properties of the pigments.
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Other notable techniques include pulsed electric field extraction, which uses short bursts of high voltage to permeabilize cell membranes, and subcritical water extraction, which utilizes water at high temperatures and pressures to solubilize pigments. Additionally, methods like ohmic heating and electric pulse technologies have been explored for their potential in disrupting cell structures to improve pigment extraction from microalgae and cyanobacteria.
Each of these techniques offers unique advantages in terms of efficiency, environmental impact, and applicability across different pigment sources and industries, reflecting the diverse and innovative approaches being developed to meet the growing demand for natural pigments.
What is the scientific evidence on the effectiveness of bromelain in treating allergic diseases?5 answersScientific evidence suggests that bromelain exhibits efficacy in managing allergic diseases. Bromelain has been found to possess anti-inflammatory properties, making it beneficial for conditions like allergen-induced inflammation. Studies highlight its role in reducing spasm, bronchial hyper-responsiveness, and inhibiting convulsions in asthma models. Furthermore, bromelain's anti-edematous, antithrombotic, and fibrinolytic effects contribute to its effectiveness in treating inflammation-based conditions. The enzyme's ability to regulate hematological, biochemical, and immunological parameters disrupted during allergic responses further supports its therapeutic potential in allergic diseases. Overall, bromelain's multifaceted properties make it a promising option for managing allergic conditions effectively.
What are the different extraction methods used for secondary metabolites?4 answersDifferent extraction methods have been developed for secondary metabolites. These methods include conventional techniques such as maceration, percolation, and Soxhlet extractions. However, innovative technologies such as high hydrostatic pressure (HHP), ultrasound (US), pulsed electric field (PEF), and supercritical fluid (SF) extraction are increasingly being used. These novel methods offer advantages such as higher extraction rates, lower impurities in the final extract, preservation of thermo-sensitive compounds, and the use of different inorganic solvents. Additionally, the use of genetic engineering techniques can enhance the production of specific secondary metabolites in plants. Optimized extraction methods, such as ultrasonication and supercritical fluid extraction, can also lead to higher yields of secondary metabolites. Overall, these various extraction methods provide options for efficiently obtaining secondary metabolites from plant sources.
What is bromelain good for?5 answersBromelain, a key enzyme found in pineapple, has multiple beneficial effects for human health. It has been found to have anti-inflammatory, immunomodulatory, antioxidant, and anticancer properties. Bromelain has been shown to exhibit cytotoxic, apoptotic, necrotic, autophagic, immunomodulating, and anti-inflammatory effects in cancer cells and animal models. It also has fibrinolytic, anti-edematous, antithrombotic, and anti-inflammatory effects, making it useful in wound debridement, improved drug absorption, and the management of sinusitis, bronchitis, angina pectoris, surgical trauma, and thrombophlebitis. Additionally, bromelain has been found to have cardiovascular benefits by decreasing hyperlipidemia, aortic inflammation, and oxidative stress, leading to atherosclerosis retardation. Bromelain has also been studied for its antibacterial and antiviral properties, showing strong binding to food-borne bacterial pathogens and SARS-CoV-2 virus targets, suggesting its potential in controlling foodborne pathogen toxicity and COVID-19. Overall, bromelain has a wide range of potential therapeutic applications, including its use as an anti-inflammatory, immunomodulatory, anticancer, cardiovascular, antibacterial, and antiviral agent.
Does boiling pineapple extract bromelain?5 answersBoiling pineapple extract does not directly extract bromelain. The extraction methods mentioned in the abstracts involve steps such as enzymatic hydrolysis, isoelectric precipitation, ultrafiltration, and freeze-drying to obtain bromelain from pineapple peel or pineapple juice. These methods focus on preserving the enzyme activity and stability of bromelain, as well as removing impurities and metal ions from the extract. The abstracts do not mention boiling as a step in the extraction process. Therefore, it can be inferred that boiling pineapple extract is not a common method for extracting bromelain.