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Is there any checkpoint for polyketides to check that the right polyketide is synthesised?

Synthetic biology

Carboxylic acid

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Polyketides have a checkpoint to ensure the right polyketide is synthesized. The biomimetic synthesis of aromatic polyketides involves catalyst-controlled transannular cyclization cascades, leading to polyketides with a defined folding and oxygenation pattern . Additionally, an iterative method for synthesizing polyketides utilizes decarboxylative dehydration condensation, which allows for the regeneration of the carboxylic acid functionality for the next elongation step . Furthermore, advancements in computational biology, metabolic engineering, structural biology, and enzymology-guided approaches have facilitated improved access to designer polyketides, ensuring the synthesis of target polyketides . The biosynthesis of polyketides is controlled by polyketide synthases (PKSs), which catalyze the condensation of thioesters and incorporate β-branches. The selectivity exerted by PKSs ensures β-branching fidelity, contributing to the synthesis of the correct polyketide .

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Open access•Journal Article•DOI Synthetic biology enabling access to designer polyketides. Alexandra A Malico, Lindsay Nichols, Gavin J. Williams - Show less +2 more 01 Oct 2020-Current Opinion in Chemical Biology 16 Citations	The paper does not specifically mention a checkpoint for polyketides to ensure the right polyketide is synthesized.
Open access•Journal Article•DOI The Stereocontrolled Total Synthesis of Polyketide Natural Products: A Thirty-Year Journey Tegan P. Stockdale, Nelson Y. S. Lam, Nelson Y. S. Lam, Matthew J. Anketell, Ian Paterson - Show less +4 more 15 Feb 2021-Bulletin of the Chemical Society of Japan 15 Citations	The paper does not mention any specific checkpoint for verifying the synthesis of polyketides. The paper focuses on the stereocontrolled total synthesis of polyketide natural products.
Open access•Journal Article•DOI Catalyst‑Controlled Transannular Polyketide Cyclization Cascades: Selective Folding of Macrocyclic Polyketides Felix C. Raps, Vincent C. Fäseke, Daniel Häussinger, Christof Sparr - Show less +3 more 20 Aug 2020-Angewandte Chemie 3 Citations	The paper does not mention any specific checkpoint for checking the synthesis of the right polyketide.
Journal Article•DOI Biomimetic iterative method for polyketide synthesis. Kengo Akagawa, Kazuaki Kudo - Show less +1 more 01 Aug 2017-Chemical Communications 10 Citations	The paper does not mention any specific checkpoint for checking the synthesis of the right polyketide.

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