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Isolation and identification of herbal 


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Isolation and identification of bioactive compounds from herbal sources are crucial steps in drug development . Herbal medicines have been utilized for centuries, offering potential alternatives to synthetic drugs due to their perceived lower side effects . The complex nature of compounds in medicinal plants necessitates extraction, isolation, and structural elucidation processes to identify potential lead molecules for therapeutic purposes . Advanced technologies like Fourier Transform Infrared Spectroscopy (FTIR) play a significant role in characterizing and identifying phytochemicals from herbal sources, aiding in the efficient analysis of bioactive compounds . These methods enable the rapid extraction and analysis of phytochemicals, providing a basis for further research on bioactivities, structure-activity relationships, and structural modifications of active compounds .

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Open accessBook ChapterDOI
Weisheng Feng, Meng Li, Zhiyou Hao, Jingke Zhang 
15 Jul 2019
18 Citations
The paper discusses analytical methods for isolating and identifying phytochemicals from plants, essential for studying bioactivities, structure-activity relationships, and structural modifications of herbal compounds.
Identification of bioactive compounds from herbal medicines is crucial for drug development. Fourier Transform Infrared Spectroscopy (FTIR) is vital for characterizing and identifying these compounds in herbal medicines.
Book ChapterDOI
01 Jan 2022
The purification of herbal biomolecules involves methods like UV-Vis spectroscopy, FTIR, GC analysis, and HPLC-ESI-MS, crucial for isolating and identifying potential therapeutic agents from herbal plants.
Efficient protocols for extraction, isolation, and structural determination of active compounds from medicinal plants are available, facilitating the identification of herbal constituents.

Related Questions

Isolation and identification of major component5 answersThe isolation and identification of the major component were successfully achieved in the study of the root of the Potentilla chinensis plant. Through various extraction methods using different solvents, the main substance was isolated and identified as compound (1) using NMR and mass analysis. Similarly, in a different study, an isolation monitoring identification system was developed, incorporating eccentric lenses, polarization components, a light field camera, a light baffle plate, and an image processor to analyze and judge multiple images effectively. Additionally, a circuit assembly was designed with conductive and non-conductive contacts to detect the configuration of hardware components, showcasing the identification capabilities of the system.
Isolation of ursolic acid3 answersUrsolic acid has been isolated from various plant species in different regions. For example, it has been isolated from the leaves of Neolamarckia cadamba using ultrasonic waves, resulting in a good yield. It has also been isolated from the leaves of Arbutus pavarii, an endemic Libyan medicinal plant, through a cytotoxicity assay-guided isolation process. In addition, ursolic acid has been obtained from Clinopodium revolutum in Peru, and a quantitative analysis for rosmarinic acid and triterpenic acids has been conducted in aqueous infusions of these plants. Furthermore, ursolic acid has been isolated from the leaves of Ocimum lamiifolium in Ethiopia using column chromatography. Lastly, it has been isolated from the leaves of Knoxia corymbosa, a species not previously known to contain this compound.
What id medicinal plant?5 answersA medicinal plant refers to a variety of plants used in herbalism for therapeutic purposes. These plants have active components with therapeutic properties and are used to treat diseases or illnesses. They have been used since ancient times and are still widely used today. Medicinal plants are found in various regions, including the Western Ghats region of India. They have significant antibacterial, antifungal, anticancer, and anti-inflammatory properties and are used to combat chronic diseases and infections. Medicinal plants are an important source of prevention against various diseases and are used as a substitute for medicine in underdeveloped countries. They are also used in traditional medicine, and the World Health Organization reports that a large percentage of the population depends on traditional medicine for their healthcare needs, with plant extracts being a common form of treatment. One example of a medicinal plant is Cucumis sativus L., which is used in traditional Unani medicine to treat ulcers.
Isolation and identification of potential antibiotic4 answersIsolation and identification of potential antibiotics was conducted in several studies. Ghadigaonkar and Rath developed a systematic protocol for the enumeration of antibiotic-resistant bacteria and determination of resistance in freshwater samples. Sitotaw et al. investigated the antibiotic production potential of bacteria isolated from a waste dumpsite and identified 26 isolates with antimicrobial activity. A study by Uwalaka et al. isolated and characterized antibiotic-producing bacteria from soil samples collected from an abattoir, identifying nine bacterial species with antimicrobial activity. In another study, cyanobacteria isolated from soil samples showed antibiotic production potential, with Nostoc muscorum demonstrating the highest inhibition activity. These studies highlight the potential of various bacterial isolates from different environments to produce antibiotics with antimicrobial activity.
Isolation of bioactive compound from the plants of mucuna utilis3 answersIsolation of bioactive compounds from the plants of Mucuna utilis has not been mentioned in the provided abstracts.
From which plants have terpenes and flavonoids been isolated and purified?5 answersTerpenes and flavonoids have been isolated and purified from various plants. Salvia apiana (white sage) is a plant that contains diterpenes, flavonoids, and triterpenes, including compounds such as rosmadial, carnosol, and cirsimaritin. Tagetes lucida (Mexican marigold) also contains compounds such as quercetagetin 7-O-β-(6''-Protocatecoyl) glucopyranoside. Additionally, terpenes and flavonoids have been isolated from Malagasy plants such as Cussonia vantsilana, Helichrysum gymnocephalum, Phyllarthron madagascariense, Gomphocarpus fructicosus, Cinnamosma sp., and C. fragrans. Furthermore, the extraction of terpenes and flavonoids from Cannabis plants has been studied, suggesting their potential synergistic effects with cannabinoids.

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