Isolation of agrobacterium rhizogenes
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Agrobacterium rhizogenes, a bacterium causing hairy root disease, is isolated for various purposes. One method involves utilizing enzyme solutions like Cellulase YC and Pectolyase Y23 to isolate protoplasts from genetically modified hairy root tissues of Brassica oleracea L. induced by A. rhizogenes strain (ATCC13332) . Another approach suggests bacterium-free production of genetically modified roots through biolistic transformation of leaf explants with specific genes like rolA, rolB, rolC, and rolD, crucial for hairy root growth . Additionally, Agrobacterium strains are isolated from different leguminous plants based on morphological, biochemical, and pathogenicity tests, identifying them as A. rhizogenes . These studies highlight diverse methods employed to isolate and characterize A. rhizogenes for genetic modification and research purposes.
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01 Apr 2017 | Not addressed in the paper. |
| Agrobacterium rhizogenes was isolated from nodules of Pisum sativum, Sesbania rostrata, Vigna mungo, V radita, and V ungiculata, characterized based on morphology, biochemistry, culture, and pathogenicity tests. | |
| Not addressed in the paper. | |
| Protoplast isolation method from genetically modified cauliflower hairy roots induced by Agrobacterium rhizogenes (ATCC13332) was successful, demonstrating the vector's ability to transfer genes into plants. | |
| Protoplast isolation method from cauliflower hairy roots induced by Agrobacterium rhizogenes (ATCC13332) was successful, demonstrating the vector's ability to transfer rol B and rol C genes. |
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