Which media are use to Isolation of Agrobacterium rhizogenes ?
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Agrobacterium rhizogenes was isolated using yeast extract mannitol agar (YEMA) medium . The isolation of Agrobacterium rhizogenes was also carried out using morphological, biochemical, cultural, and pathogenicity tests .
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Patent 16 Jun 2010 1 Citations | The paper does not mention the media used for the isolation of Agrobacterium rhizogenes. The paper is about a chinar tissue culture method suitable for Agrobacterium rhizogenes mediation. |
Open access•Journal Article | The paper does not mention the specific media used for the isolation of Agrobacterium rhizogenes. |
Open access 01 Jan 2011 | The paper does not mention the specific media used for the isolation of Agrobacterium rhizogenes. |
Open access 01 Jan 2010 | The paper mentions that Agrobacterium rhizogenes was isolated from the root nodules of leguminous plants using yeast extract mannitol agar (YEMA) as the isolation media. |
| The paper does not mention the specific media used for the isolation of Agrobacterium rhizogenes. |
Related Questions
Isolation of agrobacterium rhizogenes5 answersAgrobacterium rhizogenes, a bacterium causing hairy root disease, is isolated for various purposes. One method involves utilizing enzyme solutions like Cellulase YC and Pectolyase Y23 to isolate protoplasts from genetically modified hairy root tissues of Brassica oleracea L. induced by A. rhizogenes strain (ATCC13332). Another approach suggests bacterium-free production of genetically modified roots through biolistic transformation of leaf explants with specific genes like rolA, rolB, rolC, and rolD, crucial for hairy root growth. Additionally, Agrobacterium strains are isolated from different leguminous plants based on morphological, biochemical, and pathogenicity tests, identifying them as A. rhizogenes. These studies highlight diverse methods employed to isolate and characterize A. rhizogenes for genetic modification and research purposes.
Isolation and characterization of agrobacterium rhizogenes from root nodules of some leguminous plants5 answersAgrobacterium rhizogenes was successfully isolated and characterized from root nodules of various leguminous plants like Pisum sativum, Sesbania rostrata, Vigna mungo, V. radita, and V. ungiculata. The isolation process involved using yeast extract mannitol agar (YEMA) medium to culture the bacteria from the nodules. The identification was based on morphological, biochemical, cultural, and pathogenicity tests, confirming the presence of A. rhizogenes in the isolates. Biochemical analyses revealed variations in exo-polysaccharide, glycogen, total protein, amino acids, and lipids among the different agrobacterial isolates from the leguminous plants. Additionally, the review emphasized the importance of characterizing the isolated bacteria through gram staining, KOH tests, and pathogenic assays like carrot disk bioassay and potato disc tests.
What is the role of agrobacterium rhizogenes in the growth and development of leguminous plants?5 answersAgrobacterium rhizogenes, a type of plant growth-promoting rhizobacteria (PGPR), plays a crucial role in the growth and development of leguminous plants. These bacteria establish a symbiotic relationship with the roots of legumes, promoting various mechanisms that enhance plant productivity. Agrobacterium rhizogenes aids in nitrogen fixation, phosphate solubilization, phytohormone production, and siderophore activity, all of which contribute to improved nutrient uptake and overall plant health. Additionally, the presence of these beneficial rhizobacteria can lead to increased root and shoot growth, vigor, biomass production, and early bloom in leguminous crops, ultimately boosting their yield and resilience to environmental stressors. Overall, Agrobacterium rhizogenes plays a vital role in sustainable agriculture by supporting the growth and development of leguminous plants through various beneficial mechanisms.
What are the different types of Rhizotron media that can be used to study fungi-plant interactions?4 answersDifferent types of media can be used to study fungi-plant interactions in rhizotrons. PDA (Potato Dextrose Agar) is a commonly used medium that yielded the highest number of genera isolated from cucumber plants and their rhizosphere soil. MRBA (Modified Rose Bengal Agar) and CZA (Czapek-Dox Agar) are also effective media for isolating fungi from roots and rhizosphere soil, respectively. MEA (Malt Extract Agar) is specifically recommended for isolating endophytic Trichoderma strains. These media provide suitable conditions for the growth and isolation of different fungal genera, allowing researchers to study their interactions with plants in rhizotron experiments.
Who 1st isolation of Agrobacterium rhizogenes?5 answersThe first isolation of Agrobacterium rhizogenes was reported by S. Murugesan et al..
What are the most important characteristics of PGPRs isolated from rice rhizosphere?4 answersPGPRs isolated from rice rhizosphere have several important characteristics. They have the ability to solubilize phosphorous and produce indole acetic acid (IAA). Some strains of PGPRs isolated from rice rhizosphere, such as PGP2, PGP5, PGP7, PGP8, PGP9, and PGP10, have shown promising potential for promoting plant growth and sustainable agriculture. These PGPRs can also enhance plant nutrition, regulate phytohormones, and suppress disease-causing organisms. Additionally, PGPRs can protect plants from diseases through various mechanisms such as antibiosis, induction of systemic resistance, and production of lytic enzymes. Bacillus and Pseudomonas spp. are predominant genera of PGPRs isolated from rice rhizosphere and have been implicated in biocontrol due to their effective competitive interactions with pathogens. Overall, PGPRs isolated from rice rhizosphere have the potential to improve plant growth, enhance nutrient uptake, and protect plants from diseases.