Bacillus subtilis from freshwater sediment?5 answersBacillus subtilis has been isolated from freshwater samples in the Rosetta branch of the Nile River in Egypt. Additionally, Bacillus isolates, including B. subtilis, have been studied for their effectiveness in bioremediation to eliminate aquatic pollution in aquaculture activities. Furthermore, the efficacy of PCR-RFLP analysis has been demonstrated for the identification of Bacillus spp. isolated from freshwater pond ecosystems, showing that this method is suitable for discrimination and identification of Bacillus species, including B. subtilis. These findings collectively highlight the presence and potential applications of Bacillus subtilis in freshwater environments, showcasing its role in both natural ecosystems and biotechnological processes for environmental remediation.
Isolation of bacillus from fermented soybean food4 answersBacillus strains have been successfully isolated from various fermented soybean products. Studies have identified Bacillus siamensis FVP1 from fermented vegetables, Bacillus licheniformis B4 from camel feces, Bacillus amyloliquefaciens from termite guts, and Bacillus velezensis DP-2 from Douchi, a fermented Chinese food. These Bacillus strains exhibited probiotic potential and the ability to enhance the nutritional value of soybean meal through solid-state fermentation. They were found to reduce allergenic proteins, anti-nutritional factors, and increase the content of essential amino acids, phenolic compounds, and antioxidant properties. Additionally, these strains showed antibacterial activity against various pathogens, highlighting their potential application in functional food supplements.
Isolation of Bacillus ?5 answersIsolation of Bacillus bacteria has been studied in various contexts. One study focused on isolating Bacillus bacteria with the potential to produce cellulase from soil samples. Another study isolated Bacillus megaterium, a spore-forming bacterium, and discussed its physiology and commercial products. Additionally, isolated Bacillus strains were used to enhance crop growth and yield. In the field of animal husbandry, Bacillus-based probiotics were isolated from chickens and chicken environments, with the aim of using them as potential probiotics in poultry production. Lastly, Bacillus species isolated from the rhizosphere soil of tomato plants were found to have plant growth-promoting abilities, including the production of indole acetic acid, phosphate solubilization, and biocontrol activity against pathogens.
Isolation of the bacillus sp from soil and waterwith temperature, days5 answersStep 1:
Bacillus sp. were isolated from soil samples, and their enzyme production was studied at 37°C. The isolates showed varying enzyme activities after different days of incubation, with some strains exhibiting high proteolytic activity. Additionally, the genetic diversity among the Bacillus sp. isolates was analyzed, and the isolates were divided into different groups based on their genetic profiles. The isolates also showed potential for industrial applications^[Xavier]^[Singh]^[Soundra Josephine].
Step 2:
1. "Isolated Bacillus strains BP1 and BP2 were a good source of protease as they also showed high proteolytic activity".
2. "Bacillus sp isolated from local soil samples collected from Maharani Science College Campus, Bangalore produced protease enzyme".
3. "The microorganisms isolated were subjected to standard morphological methods for presumptive identification of the genus Bacillus".
Step 3:
Bacillus sp. were isolated from soil samples, and their enzyme production was studied at 37°C ^[Soundra Josephine]. The isolates showed varying enzyme activities after different days of incubation, with some strains exhibiting high proteolytic activity ^[Singh]. Additionally, the genetic diversity among the Bacillus sp. isolates was analyzed, and the isolates were divided into different groups based on their genetic profiles ^[Xavier]. The isolates also showed potential for industrial applications ^[Soundra Josephine].
Does bacillus subtilis have the potential in degrading crude oils?5 answersBacillus subtilis has shown potential in degrading crude oils. Studies have demonstrated that defined co-cultures of Bacillus subtilis with other bacterial strains, such as Pseudomonas aeruginosa, can significantly improve the degradation efficiency of crude oil, enhancing it from 32.61% and 54.35% in individual cultures to 63.05% in co-cultures. Additionally, newly isolated strains of Bacillus subtilis have been found to have a high capability for crude oil abatement, with degradation rates ranging from 70.0% to 83.5% in liquid media. Furthermore, Bacillus subtilis strains have been shown to produce surfactin, a surface-active compound with emulsification activity that can decrease the surface tension of water and interfacial tension of crude oil. These findings suggest that Bacillus subtilis has the potential to be used in bioremediation strategies for crude oil-contaminated environments.
What is the use of bacillus subtilis in biotechnology?1 answersBacillus subtilis is widely used in biotechnology due to its ability to secrete large amounts of proteins and produce commercially interesting compounds. It is a model organism for studying bacterial cell biology and has been extensively used for genetic manipulation and investigation. B. subtilis has a fully sequenced genome and has been the subject of genome- and proteome-wide studies, providing important insights into its biology. The bacterium is also valuable for industrial applications, such as vitamin production and concrete healing. Additionally, B. subtilis spores have unique characteristics, including extreme resistance to environmental stresses, making them a novel platform for various applications, such as drug delivery and material sciences. The establishment of genetic code expansion systems in B. subtilis has enabled greater understanding and control of proteins, further enhancing its potential for biotechnological applications.