Phosphate buffer prepared from KH2PO4 and Na2HPO4 has a buffering capacity at what pH limit?
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Phosphate buffer prepared from KH2PO4 and Na2HPO4 exhibits buffering capacity within a pH range of 6.0 to 8.0 . This buffer solution is commonly utilized in biological research due to its ability to maintain a constant pH, with a typical formulation for 1x PBS including 137 mM NaCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4 at a pH range of 7.2 to 7.4 . The stability of hydroxyapatite-TiO2-Ti structures in buffer solutions of KH2PO4-Na2HPO4 at different pH levels (6.0, 7.0, and 8.0) further confirms the effectiveness of phosphate buffers in maintaining pH within this range . Therefore, the buffering capacity of phosphate buffer prepared from KH2PO4 and Na2HPO4 is optimal within the pH limits of 6.0 to 8.0.
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| Papers (5) | Insight |
|---|---|
| The paper does not address the buffering capacity of a phosphate buffer prepared from KH2PO4 and Na2HPO4. | |
| Phosphate buffer prepared from KH2PO4 and Na2HPO4 has a buffering capacity within pH 3 to 12, as indicated in the research on anthocyanin stability in red dragon fruit peel extract. | |
| Phosphate buffer prepared from KH2PO4 and Na2HPO4 has a buffering capacity within the pH range of 6.0 to 8.0, as studied in the stability of hydroxyapatite-TiO2-Ti structures. | |
| Phosphate buffer prepared from KH2PO4 and Na2HPO4 has a buffering capacity within the pH range of 5.8 to 7.2. | |
| Phosphate buffer prepared from KH2PO4 and Na2HPO4 has a buffering capacity within the pH range of 7.2 to 7.4, as per the provided PBS solution formulation. |
Related Questions
How to form the phosphate buffer?5 answersTo form a phosphate buffer solution, various methods and compositions are available. One method involves using sodium dihydrogen phosphate, disodium hydrogen phosphate, and sodium chloride in specific concentrations to create a buffer with low osmotic pressure. Another approach includes utilizing phosphoric acid, sodium sulfate, phosphorus pentoxide, sodium hydroxide, sodium chloride, protamine, dimethyl sulfoxide, and water, where protamine enhances stability and longevity of the buffer solution. Additionally, a protocol for making pH=6.0 phosphate buffer is outlined for applications like preparing worm media, such as NGM. Furthermore, a buffered phosphorus solution containing phosphorus compounds and an organic base can serve as a fertilizer or fungicide. These diverse methods and compositions cater to different needs and applications requiring phosphate buffer solutions.
How to prepare phosphate buffer of different pH?5 answersPhosphate buffers of different pH can be prepared by adjusting the concentration of phosphate ions and the pH of the solution. The electrochemical behavior of Cu in different phosphate buffers was studied, and it was found that at pH 6.0 and 8.0, the passivation of Cu is due to the anodic formation of a basic Cu(II) phosphate. In borate and phosphate buffer solutions at pH 8.4, the reduction of the passive film on iron leads to different reactions. In borate buffer, the passive film is completely dissolved, resulting in soluble Fe(2+), while in phosphate buffer, the reduction product is metallic iron. Amorphous calcium-magnesium phosphates were prepared by precipitation from moderately supersaturated aqueous solutions at pH 7, and the proportion of protonated phosphate ions increased with the magnesium to calcium ion activity ratio. The pH of buffer solutions without chloride ions and with saline media of ionic strength I = 0.16 molkg-1 can be calculated and determined for use as standards in the physiological pH range.
What is the pH of a buffer solution?5 answersA buffer solution is a solution that resists changes in pH when an acid or base is added. The pH of a buffer solution can vary depending on the specific buffer system used. The pH range of buffer solutions mentioned in the abstracts is between 3.07 and 9. The pH value of a buffer solution is important because it determines the effectiveness of the buffer in maintaining a stable pH. Different buffer systems have different optimal pH ranges for their function. For example, one abstract mentions a Tris buffer solution with a pH range of 7 to 9. Another abstract mentions buffer solutions with a pH range of 5 to 9. Therefore, the pH of a buffer solution can vary depending on the specific buffer system and its intended application.
Can phosphate buffer saline be supporting electrolyte in an electrochemical solution?4 answersPhosphate buffer saline (PBS) can be used as a supporting electrolyte in electrochemical solutions. It has been shown to be effective in maintaining the stability and performance of electrochemical devices, such as microbial electrolysis cells (MECs). In MECs, a combo buffer with a mixture of phosphate and bicarbonate ions has been found to be feasible, with large replacement of phosphate by bicarbonate still maintaining current density and stability. Additionally, phosphate buffers have been commonly used in vanadium redox flow batteries (VRFBs) to improve the stability of the positive electrolyte. However, it should be noted that the stability of phosphate buffers can be affected by factors such as concentration and pH, and their breakdown can have implications on the performance of electrocatalysts. Overall, phosphate buffer saline can be a suitable supporting electrolyte in electrochemical solutions, but its specific application and performance may vary depending on the system and conditions.
How to do phosphate buffer saline?4 answersPhosphate buffer saline (PBS) can be prepared by adding sodium dihydrogen phosphate, disodium hydrogen phosphate, and sodium chloride to water for injection. The specific concentrations of these components may vary, but typically, 0.003-0.005% of sodium dihydrogen phosphate, 0.025-0.030% of disodium hydrogen phosphate, and 0.80-0.85% of sodium chloride are added. The mixture is stirred at a low rotation speed, and after stirring is completed, the solution is sterilized and filtered to obtain the phosphate buffer solution. Another method involves adding phosphoric acid and sodium hydroxide to deionized water simultaneously but at different speeds, resulting in an aqueous solution of disodium phosphate and monosodium phosphate in a proportion of 1:2.
What is percentage of phosphate content in h2po4?5 answersThe percentage of phosphate content in H2PO4 varies depending on the specific compound or solution being studied. In the context of the papers provided, the percentage of phosphate content in H2PO4 is not explicitly mentioned. However, the papers discuss the use of Mg(H2PO4)2, KH2PO4, Al(H2PO4)3, and H2PO4- ions in different experimental setups and reactions. These papers provide information on the properties, structures, and reactions involving these compounds, but do not specifically mention the percentage of phosphate content in H2PO4.