Scanning Ion cunductence Microscopy to study ion channels?
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Scanning Ion Conductance Microscopy (SICM) has evolved as a pivotal tool for studying ion channels, leveraging its non-contact, high-resolution imaging capabilities to explore cellular and subcellular structures. The integration of SICM with gated ion channels into probes offers a novel approach for spatially resolved sensing at interfaces, as demonstrated by the use of dual-barrel pipettes where one barrel controls the pipette position and the second houses voltage-gated ion channels. This configuration allows for the imaging of local electric field gradients that activate TRPV1 channels near pores, showcasing the potential for ion channel-based sensing in imaging applications. Moreover, the development of dual-barrel membrane patch-ion channel probes (MP-ICPs) integrated into SICM systems has been shown to provide a revolutionary method for spatially resolved chemical sensing. This approach has been validated with different types of ion channels, including transient receptor potential vanilloid 1 and large conductance Ca2+-activated K+ channels, establishing the versatility of the method. The interplay between the SICM barrel and the ion channel probe (ICP) barrel has been studied, revealing that channel activity can be modulated by the SICM barrel potential, depending on the charge of the ligands gating the ion channels. Furthermore, the use of dual-barrel ion channel probes (ICPs) consisting of an open barrel for SICM imaging and a second barrel with a membrane patch for investigating ion channel activities has been described. This setup allows for the independent addressing of each barrel, enabling the study of the influence of ligand proximity on ion channel activation. These advancements underscore the utility of SICM in the study of ion channels, offering insights into their spatial distribution and activity, and opening new avenues for the investigation of cellular processes at the nanoscale.
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| Papers (10) | Insight |
|---|---|
15 Citations | Scanning Ion Conductance Microscopy (SICM) coupled with dual-barrel ion channel probes allows non-invasive imaging and investigation of ion channel activities, particularly demonstrating the influence of probe-substrate distance on channel activations. |
19 Citations | Scanning Ion Conductance Microscopy (SICM) integrated with Membrane Patch-Ion Channel Probes enables spatially resolved chemical sensing and single ion channel activity measurements, revolutionizing ion channel studies. |
| Scanning Ion Conductance Microscopy (SICM) is utilized for single cell analysis, investigating single channels and receptors in cellular membranes, and delivering biomolecules to precise locations within cell cultures. | |
4 Citations | Not addressed in the paper. |
| Not addressed in the paper. | |
20 Feb 2022 | Not addressed in the paper. |
| Scanning Ion Conductance Microscopy can be utilized to study mechanosensitive ion channels non-invasively, enabling contactless stimulation and examination of individual ion channels without damaging the nanopipette functionality. | |
7 Citations | Scanning Ion Conductance Microscopy (SICM) utilizes gated ion channels like TRPV1 for spatial sensing at interfaces, demonstrating a robust platform for imaging applications with ion channels. |
| Scanning Ion Conductance Microscopy (SICM) is utilized for studying interfaces in biology and materials science, offering versatile biophysical and electrochemical measurements, but not specifically for studying ion channels. | |
| Not addressed in the paper. |
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