Studies Using culture Media for human sperm preparation?
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Studies have been conducted on the use of culture media for human sperm preparation. These studies have explored the efficiency of synthetic protein-free media (PFM) compared to commercial protein-containing media (CPC) and minimum essential medium+HSA (MEM) in spermatozoa washing and preparation procedures. The results indicate that PFM is equally efficient as CPC and MEM in maintaining spermatozoa motility, vitality, membrane integrity, and DNA fragmentation levels over short periods in vitro . Additionally, the use of synthetic PFM has been shown to retain spermatozoa activity at room temperature for up to 24 hours, with the highest retention observed at 22°C . These findings suggest that synthetic PFM can be a viable alternative to commercial protein-containing media for human spermatozoa washing and preparation, potentially improving the outcomes of assisted reproduction technology (ART) procedures .
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Open access•Dissertation 01 Jan 2016 8 Citations | The paper discusses the efficacy of synthetic chemically defined protein-free spermatozoa handling and culture media on spermatozoa motility and vitality. It concludes that the protein-free media is equally efficient as conventional protein-containing media for human sperm preparation. |
| The paper discusses the effects of synthetic serum supplementation on sperm capacitation and function test results, but it does not specifically mention studies using culture media for human sperm preparation. | |
23 Mar 2018 | The paper discusses the use of a commercially available dual-buffer solution of HEPES and MOPS called Multipurpose Handling Medium (MHM) for human sperm handling. It demonstrates that MHM performs better than single buffer controls in terms of sperm viability, motility percent, and rapid progression. |
| The paper discusses the efficiency of synthetic protein-free media, minimum essential medium+HSA (MEM), and commercial protein-containing medium (CPC) in spermatozoa washing and preparation. |
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