What types of tape are most commonly used for TEM sample preparation and why?4 answersThe most commonly used types of tape for Transmission Electron Microscope (TEM) sample preparation are those that allow for successful sample transfer and manipulation. In the field of semiconductor manufacturing, where TEM analysis is crucial, techniques such as in-situ lift-out and optical microscopy-based micro-manipulation systems have been employed for safe and efficient sample preparation. These methods emphasize the importance of proper selection of coating materials, tilted angle deposition, in-situ lift-out techniques, and control of electrostatic forces to transfer samples onto TEM grids effectively. Additionally, for studying heterogeneous catalyst extrudates, electron-transparent thin sections are prepared to preserve the structure and composition of the samples, enabling detailed elemental analysis critical for understanding catalyst performance.
What are the best ways to microscope in the laboratory?5 answersThe best ways to microscope in the laboratory include using a microscope laboratory system with an image sensing unit, A/D conversion unit, DSP unit, storage unit, controller, and sensor for real-time image display and image purification. Another effective method is using a laboratory-size three-dimensional water-window x-ray microscope with condenser and objective grazing incidence Wolter type I mirrors, an electron-impact-type x-ray source, and a back-illuminated CCD for high-resolution imaging of biological samples. Additionally, a Full-Field Laboratory Transmission X-ray Microscope (L-TXM) operated with a nitrogen laser plasma source and objective zone plates can achieve high-resolution imaging with reduced exposure times, enabling cryo tomography in a laboratory environment. Furthermore, a laboratory-size three-dimensional water-window x-ray microscope combining wide-field transmission x-ray microscopy with tomographic reconstruction techniques can provide high-resolution imaging of fine structures. Lastly, an open-source, 3D-printed, and fully-automated laboratory microscope with motorized sample positioning and focus control offers customizability and accessibility for low-volume manufacturing and maintenance.
What are the advantages and disadvantages of the different methods for specimen preparation for fiber metal laminate?5 answersDifferent methods for specimen preparation for fiber metal laminate have their own advantages and disadvantages. One method involves using a modular, process-inspired test setup for infiltration experiments, which allows for permeability measurements and good repeatability of results. Another method involves a surface treatment process for titanium alloy sheets, which improves inter-laminar strength and bonding with epoxy resin. A hot press method is used for composite molding of fiber prepreg tape fabrics and metal sheets, resulting in a smooth and flat workpiece with high mechanical performance and reduced weight. A thermoplastic blended woven fabric is used in another method, which improves interlayer bonding force and mechanical properties of the fiber metal laminate. The advantages of these methods include good repeatability, improved inter-laminar strength, reduced weight, and improved interlayer bonding force. However, each method may have limitations such as the need for specialized equipment or specific material requirements.
How to prepare corrosion specimen before surface analysis with SEM?3 answersCorrosion specimens for surface analysis with SEM can be prepared using various techniques. One common method involves filling the internal luminal system or space of the specimen with a liquid medium that solidifies in situ. The surrounding tissue is then corroded and the resulting replica is dried, rendered conductive, and examined in the SEM. Another approach involves cleaning the specimen to remove any hydrocarbon contamination and providing a conductive coating, especially for insulators. Solvent cleaning, degreasing in an ultrasonic cleaner, mechanical brushing, replica stripping, and chemical etching are some of the techniques used for surface cleaning. Additionally, combinatorial intense heavy metal staining of cells and tissues is often necessary to maximize image acquisition speed and achievable resolution, especially when the samples are embedded in nonconductive epoxy resins.
Why water is used in specimen preparation for the microscope?5 answersWater is used in specimen preparation for the microscope because it is the most abundant component of biological material and is necessary for preserving the structure and biological activity of cells and tissues. Traditional electron microscopy techniques exclude water because it evaporates rapidly under vacuum conditions. However, recent advancements in cryoelectron microscopy have allowed for the direct observation of frozen-hydrated specimens, overcoming the limitations of water evaporation. Additionally, the use of water in specimen preparation allows for the examination of specimens in their natural hydrated state, providing valuable information about the distribution and behavior of water in heterogeneous systems. Water-based techniques, such as impedance microscopy and environmental scanning electron microscopy, enable high-resolution imaging of specimens in water without the need for specific sample-preparation protocols or the risk of radiation damage.
How can I prepare my sample for scanning electron microscopy?5 answersTo prepare a sample for scanning electron microscopy (SEM), it is essential to follow proper sample preparation techniques. Flawed sample preparations can lead to inaccurate results and false conclusions. Researchers, postgraduate students, and technicians in the life sciences should have the necessary knowledge to prepare samples for SEM investigations. Sample preparations involve steps such as transferring fixed tissues to a slide glass, cutting the tissues to the desired size, cleaning and dehydrating the tissue, embedding the tissue, and accurately trimming and positioning the sample for observation. Additionally, desiccated samples can be imaged directly, while nonconductive samples may require coating with an electron conductive film using a gold sputter coater. Proper sample preparation ensures accurate and reliable SEM imaging, allowing for detailed analysis of the sample's composition and topography.