What are the criteria for ORF to be protein coding?
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Open reading frames (ORFs) are considered protein-coding if they meet specific criteria. These criteria include the size of the ORF, with small ORFs (smORFs) and short ORFs (sORFs) being identified as potential protein-coding regions . Additionally, the presence of coding potential within the ORF is determined through ribosome profiling and other advanced techniques . Evaluation methods for predicting protein-coding potential of ORFs involve assessing sequence-based algorithms and utilizing random sequence strategies for prediction accuracy . It is crucial to differentiate between exonic regions that are protein-coding and those that are non-coding, emphasizing the importance of clear language in genetic studies . Overall, the identification and validation of protein-coding ORFs involve a combination of bioinformatics, omics approaches, and functional characterization techniques .
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| Papers (4) | Insight |
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1 Citations | Not addressed in the paper. |
| Small open reading frames (smORFs) are considered protein-coding if they show constraint within human populations, evolutionary conservation, and enrichment among disease-associated variants, as per the research findings. | |
| Short open reading frames (sORFs) ≤ 300 bases, encoding microproteins ≤ 100 amino acids, can be identified as protein-coding using ribosome profiling (RIBO-Seq) to detect sORF transcripts. | |
3 Citations | The criteria for ORF to be protein coding include short sequence, low expression level, and challenges in genome annotation due to their small size and limited prediction methods. |
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