What are the most commonly used methods to assess Glyoxalase 2 enzyme?
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The most commonly used methods to assess Glyoxalase 2 enzyme are spectrophotometric measurements and metal analyses. Spectrophotometric measurements are used to determine the activity of Glyoxalase 2 by following the decrease in A240, which corresponds to the change in molar absorption coefficient Δe240=-3.10 mM⁻¹·cm⁻¹ . Metal analyses are used to characterize the nature of the metal center in Glyoxalase 2. These analyses involve determining the concentrations of zinc, iron, and cobalt in the enzyme .
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4 Citations | The provided paper does not mention the commonly used methods to assess Glyoxalase 2 enzyme. |
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78 Citations | The paper does not mention the most commonly used methods to assess Glyoxalase 2 enzyme. The paper is about the measurement of glyoxalase activities. |
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