M
Michael W. Crowder
Researcher at Miami University
Publications - 121
Citations - 4147
Michael W. Crowder is an academic researcher from Miami University. The author has contributed to research in topics: Active site & Substrate (chemistry). The author has an hindex of 36, co-authored 117 publications receiving 3763 citations. Previous affiliations of Michael W. Crowder include Medical College of Wisconsin & University of Virginia.
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Journal ArticleDOI
Hydrolysis of phosphate monoesters: a biological problem with multiple chemical solutions
TL;DR: This work suggests that phosphatases are as important as kinases in regulatory processes, and that they constitute a diverse group of enzymes that utilize a variety of chemical means to accelerate phosphate ester hydrolysis.
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Overexpression, Purification, and Characterization of the Cloned Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia
TL;DR: The metallo-β-lactamase L1 from Stenotrophomonas maltophilia was cloned, overexpressed, and characterized by spectrometric and biochemical techniques, demonstrating that the cloned enzyme exists as a tightly held tetramer with a molecular mass of ca.
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Dipicolinic Acid Derivatives as Inhibitors of New Delhi Metallo-β-lactamase-1.
Allie Y. Chen,Pei W. Thomas,Alesha C. Stewart,Alexander Bergstrom,Zishuo Cheng,Callie Miller,Christopher R. Bethel,Steven H. Marshall,Cy V. Credille,Christopher L. Riley,Richard C. Page,Robert A. Bonomo,Robert A. Bonomo,Michael W. Crowder,David L. Tierney,Walter Fast,Seth M. Cohen +16 more
TL;DR: A new class of inhibitors for NDM-1 and two related β-lactamases, IMP and VIM-2, was identified by utilization of fragment-based drug discovery (FBDD), and was identified to be highly selective for MBLs when compared to other Zn(II) metalloenzymes.
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Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli
Liliya A. Yatsunyk,J. Allen Easton,Lydia R. Kim,Stacy A. Sugarbaker,Brian Bennett,Robert M. Breece,Ivan I. Vorontsov,David L. Tierney,Michael W. Crowder,Amy C. Rosenzweig +9 more
TL;DR: Conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn2+ substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer.
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The Continuing Challenge of Metallo-β-Lactamase Inhibition: Mechanism Matters
TL;DR: This review surveys the mechanisms of MBL inhibitors and describes methods that determine the mechanism of inhibition to guide development of future therapeutics.