What are the problems about using FBS in culture media?
Best insight from top research papers
The use of fetal bovine serum (FBS) in culture media presents several problems. Firstly, FBS is an ill-defined mixture of components with qualitative and quantitative variations, making it difficult to standardize experiments . Secondly, there are ethical concerns regarding the collection of FBS from bovine fetuses, as well as potential biosafety risks such as the presence of contaminants or adverse factors . Additionally, there is a global supply versus demand issue, with a severe geographical mismatch between the highest demand for FBS in the US and Europe and the major sources of FBS in countries like Brazil, Argentina, and South Africa . These factors have led to efforts to reduce or replace the requirement for FBS in cell culture media .
Answers from top 5 papers
More filters
| Papers (5) | Insight |
|---|---|
Open access•Journal Article 7 Citations | The problems with using FBS in culture media are not mentioned in the provided paper. |
Open access•Journal Article 1 Citations | The paper does not mention any problems specifically related to using FBS in culture media. |
| The paper mentions that using FBS in culture media for porcine umbilical cord matrix cells (PUCs) can increase the risk of microbial contamination and may not produce consistent results. | |
160 Citations | The problems with using FBS in culture media include batch-to-batch variations, potential contamination with adverse factors, ethical concerns about its collection from bovine fetuses, and concerns about the global supply versus demand. |
| The problems with using FBS in culture media include its ill-defined nature, ethical concerns regarding the collection from bovine fetuses, and concerns about the global supply vs. demand of FBS. |
Related Questions
What is the role of FBS in the growth and propagation of lentiviruses?5 answersFetal Bovine Serum (FBS) plays a crucial role in the growth and propagation of lentiviruses. FBS is commonly used as a supplement in cell culture media for lentiviral production, aiding in the efficient transduction of lentiviral sgRNA, shRNA, and protein overexpression. Additionally, FBS is essential in the lentiviral production protocol, contributing to the successful generation of lentiviral vectors. Moreover, FBS supports viral propagation and growth in cultivated cells, ensuring the production of high-quality lentiviral vectors. Its presence in the medium is vital for the inhibition of lentivirus spread within cells, highlighting its significance in lentiviral research. Therefore, FBS serves as a critical component in the growth and propagation of lentiviruses, facilitating various stages of lentiviral vector production and transduction.
How do fandoms contribute to the production and distribution of cultural content on social media?5 answersFandoms play a significant role in the production and distribution of cultural content on social media platforms. They utilize platforms like YouTube, TikTok, and Twitter to create and share content related to their interests. Fan communities engage in activities such as creating fan-made videos, managing content production, and sharing personal reflections on media content. These activities contribute to the construction of narratives, the dissemination of information, and the reinterpretation of cultural products. Through their engagement with social media, fandoms not only amplify existing content but also generate new meanings and discussions around various cultural products, thereby shaping the landscape of online cultural production and distribution.
How does Facebook usage contribute to social problems?5 answersFacebook usage contributes to social problems through various mechanisms. Research indicates that factors like loneliness, self-esteem, self-presentation, and extraversion are linked to problematic Facebook use, potentially leading to negative outcomes and impacting well-being. Additionally, the misuse of digital spaces, including social media platforms like Facebook, can result in defamation, ridicule, and unjustified lynching, highlighting the ethical implications of online interactions. Furthermore, the comparison behavior facilitated by Facebook, where users compare themselves to others, can lead to decreased self-esteem and negative feelings, especially among individuals who engage in frequent social comparisons on the platform. These findings underscore the complex relationship between Facebook usage and its potential contribution to social problems, emphasizing the need for responsible and ethical online behavior.
What are the main problems with Facebook as a brand?5 answersFacebook as a brand faces several problems. One of the main problems is the potential risk of negative user-generated content on social media platforms, which can lead to the emergence of a crisis for brands. Another problem is the lack of understanding of the effects that Facebook pages have on the perception of the brand by users. Luxury fashion brands using Facebook as a marketing tool also face challenges in maintaining brand integrity while keeping up with social media trends. Additionally, the type of commercial Facebook posts and the content of those posts can influence message credibility, attitude towards the advertised brand, and perceived purchase risk. These problems highlight the need for brands to effectively manage their corporate reputation on social media platforms and to understand the impact of Facebook on brand perception and consumer behavior.
What are the challenges towards Facebook videos?5 answersFacebook videos face several challenges. One challenge is the need to predict the future popularity of videos in order to focus intensive processing on those that will improve the quality of the most watch time. Another challenge is the limited ability to convert engagement into donations, highlighting the need for greater experimentation to understand how to raise funds effectively. Additionally, there is a need to develop strong cryptography algorithms to secure images and videos while transferring them over the network. These challenges include the worldwide surge in the use of social media, especially during the COVID-19 pandemic. Lastly, there is a need to identify and address the challenges faced by Ndau users on the Rekete ChiNdau platform.
What are the disadvantages of popular culture in social media?5 answersPopular culture in social media has several disadvantages. It can lead to the decline of traditional values and cultures, as seen in Myanmar where social media has caused a transformation in behaviors and a deviation from Myanmar traditions and cultures. Additionally, popular culture in social media can contribute to the commodification and standardization of fan culture, reducing it to mere "user-generated content" and potentially threatening its creative history. Furthermore, popular culture in social media is often criticized for being over-commercialized, presenting skewed values, and lacking cultural value. These disadvantages highlight the potential negative impacts of popular culture in social media on traditional values, fan culture, and the overall cultural landscape.