What are the separation methods of tea protein?
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The separation methods of tea protein include various techniques such as extraction with TCA/acetone solution followed by 2D-PAGE for effective separation and identification using LC/MS/MS . Another method involves utilizing tubular membranes with adsorbents for protein separation, where desorption buffer solutions are used to dissolve the adsorbed substances . Additionally, a method for separating protein from the tea oil layer includes steps like enzymatic hydrolysis and centrifugal separation to obtain the protein . Furthermore, soil proteomics research utilizes extraction with citrate, SDS, or a mixture of both, followed by 2-DE electrophoresis and LC–MS/MS identification to build a database of soil proteins and microbial species . Lastly, the preparation of oil-tea meal enzymatically-modified protein involves ultrasonic-assisted hydrolysis, compound protease hydrolysis, and centrifugal separations to obtain the final product with high protein extraction efficiency .
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| The separation methods of oil-tea meal enzymatically-modified protein include centrifugal separation and vacuum low-temperature drying, enhancing protein extraction efficiency and bioactivity. | |
| The separation method of tea protein involves soaking camellia seed powder in alcohol, centrifugal separation of oil layer, washing with water, and enzymatic treatment with phospholipase for protein extraction. | |
| The separation method for tea proteins involves using 2D-PAGE for effective separation, resulting in clear protein spots, aiding in the identification of 4,117-4,218 proteins through LC/MS/MS. | |
| The separation method of protein in the paper involves using a tubular membrane with an adsorbent to separate substances, which can be applied to separate tea protein. |
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