What are the specific internal solutions used in SH-SY5Y cells for in vitro studies?
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The specific internal solutions used in SH-SY5Y cells for in vitro studies include a lysis buffer containing 1% Triton X 100, 50 mM Tris, pH 7.5, 750 mM NaCl, 5 mM EDTA, 4 units RQ1 RNase-free DNase, and a protease and phosphatase inhibitor mix . Additionally, the insoluble pellets are resuspended in a solution containing 2% SDS, 8 M urea, 10 mM Tris, pH 7.5, 4 units RQ1 RNase-free DNase, and a protease and phosphatase inhibitor mix .
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28 Apr 2022 | The specific internal solutions used in SH-SY5Y cells for in vitro studies are 1% (v/v) Triton X 100, 50 mM Tris, pH 7.5, 750 mM NaCl, 5 mM EDTA, 4 units RQ1 RNase-free DNase, and protease and phosphatase inhibitor mix (Halt). |
23 Citations | The specific internal solutions used in SH-SY5Y cells for in vitro studies are not mentioned in the paper. The paper discusses the suitability of SH-SY5Y-derived neurons as a model system for investigating TAU sorting and neuronal vulnerability in neurodegenerative diseases. |
| The specific internal solutions used in SH-SY5Y cells for in vitro studies are not mentioned in the provided paper. The paper focuses on the characteristics of the SH-SY5Y cell line and its use as a relevant in vitro cell model for investigating neurotoxicology in humans, particularly in relation to organic pollutants. | |
| The specific internal solutions used in SH-SY5Y cells for in vitro studies are not mentioned in the provided paper. The paper focuses on investigating the SH-SY5Y cell surfaceome as a model for neuronal-targeted therapeutic modalities. | |
| The specific internal solutions used in SH-SY5Y cells for in vitro studies are not mentioned in the provided paper. |
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