What enzymes are used to release cells when propagating in tissue culture?
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Enzymes used to release cells when propagating in tissue culture include pronase , a proteolytic enzyme that is effective for dispersing primary fibroblastic cell lines. Trypsin is also commonly used as a dispersing agent for tissue culture cells, although its effectiveness may vary depending on the cell line. In addition, serine proteases or esterases have been found to be released from cell cultures into the growth medium . These enzymes can be labeled and identified using active site labeling with tritiated DFP, and their presence can be detected through SDS-polyacrylamide gel electrophoresis and scintillation autoradiography . Overall, a combination of enzymes may be used for tissue dispersal in cell culture, and the specific enzymes employed may depend on the cell type and desired outcome .
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01 Jan 1987 1 Citations | The paper does not specifically mention the enzymes used to release cells when propagating in tissue culture. |
6 Citations | The provided paper does not mention the specific enzymes used to release cells when propagating in tissue culture. |
16 Citations | The provided paper does not mention the specific enzymes used to release cells when propagating in tissue culture. |
25 Citations | The paper mentions the use of two enzymes, pronase and trypsin, for releasing cells in tissue culture. |
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