What is onpg?
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Ortho-Nitrophenyl-β-D-galactoside (ONPG) is a compound used in microbiology to detect the presence of β-galactosidase activity. It is mentioned in the context by Wu et al., where they describe an environmental Vibrio cholerae strain that is ONPG-negative . ONPG is utilized in various assays to assess enzyme activity, such as in the detection of coliforms in dairy products. Gawai et al. mention the use of ONPG in their study comparing conventional methods with chromogenic and fluorogenic assays for detecting E. coli and coliforms in milk samples . ONPG is a substrate that, upon cleavage by β-galactosidase, produces a yellow compound, ortho-nitrophenol, which can be quantified spectrophotometrically, aiding in the identification and enumeration of specific bacterial strains.
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| Papers (5) | Insight |
|---|---|
| ONPG stands for o-nitrophenyl-ß-d-galactopyranoside, used in the study as a method to quantify coliforms in milk samples, providing a reliable alternative to conventional methods. | |
| Not addressed in the paper. | |
12 Citations | Not addressed in the paper. |
| ONPG stands for ortho-nitrophenyl-β-d-galactoside. It is a compound used in microbiology to detect the presence of β-galactosidase enzyme activity, commonly found in Escherichia coli. | |
| Not addressed in the paper. |
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