What is the advantage to use shared ASV analysis compared to OTU analysis?
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Shared amplicon sequence variant (ASV) analysis offers advantages over operational taxonomic unit (OTU) analysis. Shared ASV analysis allows for a more accurate identification of oral bacterial and archaeal species, as it considers amplicon similarity/identity (ASI) values of ≥97% . This approach helps to identify distinct genera or higher taxonomic ranks that may be erroneously clustered together in OTUs . In contrast, OTU analysis using a 97% similarity threshold can lead to inaccurate descriptions of oral microbial species and impact microbial diversity parameters . Shared ASV analysis provides a more precise representation of the oral microbiota, revealing conflicting roles of certain species and highlighting associations with health and disease conditions . Therefore, shared ASV analysis improves the credibility and comparability of findings in oral microbiome research .
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| Papers (4) | Insight |
|---|---|
| The provided paper is about the feasibility of using Average Nucleotide Identity (ANI) analysis for poxvirus classification. There is no information in the paper about shared ASV analysis or OTU analysis. | |
| The provided paper is about a shared medical data analysis system and method. It does not provide any information about shared ASV analysis or OTU analysis. | |
| The provided paper does not discuss the advantage of using shared ASV analysis compared to OTU analysis. The paper focuses on evaluating primer pairs and identifying oral species that may be erroneously clustered in the same OTU. | |
| The provided paper does not mention anything about shared ASV analysis or OTU analysis. |
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