scispace - formally typeset
Search or ask a question

What is the limit of detection of OPA assay? 


Best insight from top research papers

The limit of detection of the OPA assay can be improved significantly by microwave-enhanced hydrolysis and subsequent post-column OPA derivatization. Compared to native fluorescence detection, the detection limit can be lowered by a factor between 60 and 120 depending on the amino acid composition of the protein .

Answers from top 4 papers

More filters
Papers (4)Insight
Journal ArticleDOI
75 Citations
The provided paper does not mention the OPA assay or its limit of detection. The paper discusses the limitations of water quality control methods and the use of the detection limit to censor data.
The paper does not mention the limit of detection of OPA assay. The paper is about optimizing proximity ligation assay (PLA) in Western blotting (WB) to lower the limit of detection.
The provided paper does not mention anything about the limit of detection of OPA assay.
The provided paper does not mention the OPA assay or its limit of detection.

Related Questions

Whats the detection limit of TEM10 answersThe detection limit of Transmission Electron Microscopy (TEM) is a multifaceted concept that depends on various factors including the electron dosage, the efficiency of electron detectors, and the specific analytical techniques employed. High-resolution TEM studies are often constrained by the electron dosage, with sensitive samples such as catalysts and macromolecules only preserving their structures below a threshold of 100 ē/Å^2 due to the detrimental effects of high radiation doses. The efficiency of electron detectors plays a crucial role, as advancements in electron sources and optics have highlighted the limitations of current detection systems, pushing the need for novel sensor technologies that offer improved Modulation Transfer Function (MTF) and Detective Quantum Efficiency (DQE). Analytical techniques within TEM, such as Energy Dispersive X-ray Spectroscopy (EDS) and Electron Energy Loss Spectroscopy (EELS), have their own detection limits. EELS is more sensitive for light elements, while EDS is better suited for heavy elements, with the detection efficiency of EDS significantly improved by new detector technology. Diagnostic negative staining electron microscopy, a technique used for pathogen detection, has shown detection limits of 10^6 particles per ml using direct adsorption and can be improved to 10^5 particles per ml for spores and 5 × 10^4 particles per ml for poxviruses with airfuge ultracentrifugation. Furthermore, the detection limit is also influenced by the method of sample preparation and analysis, with arbitrary detection limits potentially misstating the "true" detection limits. The concept of randomly exposing areas of the specimen to the electron beam to mitigate damage introduces challenges in immediate qualitative analysis due to the difficulty in interpreting subsampled images. Lastly, the effect of chromatic aberrations and the choice of using energy-filtered electrons can impact the detection limit, especially in the context of dark field microscopy of unstained specimens. In summary, the detection limit of TEM is not a fixed value but varies with the specific conditions of the experiment, including the type of sample, the analytical technique used, and the efficiency of the detection system.
Whats the detection limit of NANOPARTICLES TEM9 answersThe detection limit of nanoparticles using various techniques varies significantly depending on the method and conditions employed. Transmission Electron Microscopy (TEM) has been highlighted as a sensitive method for measuring nanoparticle dispersions in solutions, potentially more sensitive than dynamic light scattering for counting individual nanoparticles, including those in agglomerates. However, the specific detection limit for TEM was not directly provided in the contexts, but insights from related nanoparticle detection technologies can offer a comparative perspective. For instance, scanning electron microscopy (SEM), a technique related to TEM in its use of electron beams for imaging, has demonstrated a size measurement limit for nano gold particles between 10 nm to 20 nm under certain conditions. This suggests that TEM, with its higher resolution compared to SEM, could potentially achieve a similar or better detection limit under optimal conditions. Interferometric techniques have been developed to detect particles smaller than 20 nm by amplifying the particle signal without amplifying the background speckle, indicating the potential for high sensitivity in nanoparticle detection. Surface plasmon resonance imaging (SPRM) allows for the real-time detection of nano- and micro-particles, with a detection limit for aqueous samples potentially below 1000 particles per microliter. Nanomechanical sensing techniques have achieved detection limits in the order of tens of femtograms to single attograms, enabling the detection of liquid volumes down to the zeptoliter scale. Inductively coupled plasma mass spectrometry (ICP-MS) has faced challenges in detecting particles below 10 nm due to sensitivity limitations. Magnetic nanoparticles-aided microwave imaging has shown the potential to detect amounts of magnetic nanoparticles between 2 and 7 mg dispersed in a volume of about one cubic centimeter. While these methods vary in their approach and application, they collectively underscore the ongoing advancements in nanoparticle detection technologies, suggesting that TEM, with its high-resolution capabilities, could potentially offer a competitive detection limit, especially when optimized for nanoparticle imaging.
What are the limitations of the Bradford Assay?5 answersThe limitations of the Bradford Assay include susceptibility to interference from nonprotein sources, such as chemicals and detergents, which can affect the accuracy of protein quantification. Foaming during mixing operations can also cause unintended light scattering and interfere with spectrometric sample analysis. Additionally, the standard technique requires larger volumes of protein samples, whereas a new protocol using an opto-microfluidic platform allows quantification using smaller volumes, resulting in a lower limit of quantification. The lysis buffer used for dissolving proteins can introduce interference and affect the protein-dye chromogenic process. However, increasing the phosphoric acid content in the dye reagent can reduce interference from the lysis buffer and improve sensitivity to proteins.
What is the limit of detection for KRAS mutations using cold PCR?4 answersThe limit of detection for KRAS mutations using cold PCR varies depending on the specific technique used. One study utilized a highly multiplexed dPCR technique combined with melting curve analysis and achieved a limit of detection of less than 0.2% for all target mutations. Another study used a modified oligonucleotide with phosphoryl guanidine (PG) groups as primers and blockers in allele-specific PCR and achieved a KRAS mutation detection limit of 20 copies of mutant DNA in a proportion as low as 0.1% of the total DNA. Additionally, a study using CRISPR-Cas12a demonstrated the ability to specifically detect 0.01% mutant KRAS DNA in the presence of wild-type DNA. Therefore, the limit of detection for KRAS mutations using cold PCR can range from less than 0.2% to as low as 0.01%, depending on the specific technique employed.
What are the limitations of immunoassays in drug screening?5 answersImmunoassays have limitations in drug screening, including variable cross-reactivity, false negatives, and false positives. These limitations can affect the sensitivity and specificity of the tests, leading to potential inaccuracies in results. Immunoassays may fail to detect certain drugs, such as benzodiazepines, as shown by liquid-chromatography tandem mass spectrometry. Additionally, immunoassays used for therapeutic drug monitoring of immunosuppressants can have significant cross-reactivity with metabolites, resulting in interference and positive bias in drug concentration measurements. It is important to be cautious when interpreting immunoassay drug screens and to consider the limitations associated with these screening procedures. Mass spectrometry analysis is often required to definitively identify and confirm drugs of abuse, but it may not be readily available.
What is the limit of detection for the biotin detection method?3 answersThe limit of detection for the biotin detection method is 0.03 nM.

See what other people are reading

What is a conductive loss?
5 answers
What are the positive impact of Internet of Things (IoT) in terms of Enhanced Safety for Energy Companies?
5 answers
How does the choice of EDFA parameters affect the accuracy of the calculated results in MATLAB?
5 answers
The choice of Erbium-doped fiber amplifier (EDFA) parameters significantly impacts the accuracy of calculated results in MATLAB. Different experimental conditions, such as input signal-to-noise ratio (SNR), input power, and gain levels, influence the stability and performance of EDFAs. The gain spectra of EDFAs are affected by parameters like fiber length, signal power, pump power, and pump direction, which can further impact the accuracy of calculations. Additionally, utilizing single or multi-wavelength input sources in EDFA systems can enhance optical communication system performance, with MATLAB models accurately representing the gain dynamics and forward Amplified Spontaneous Emission (ASE). Therefore, optimizing these parameters based on experimental data is crucial for achieving precise results in MATLAB simulations.
How teperature affect PPKTP phase matching?
5 answers
Temperature significantly impacts the phase matching properties of periodically poled KTiOPO4 (PPKTP) crystals. As temperature increases from 20°C to 120°C, the group-velocity-matched and phase-matched wavelengths of biphotons generated from various PPKTP isomorphs experience notable shifts. Specifically, the phase matching temperature of PPKTP affects the squeezing capabilities of degenerate optical parametric amplifiers (DOPAs), with higher phase matching temperatures leading to decreased squeezing due to additional phase noise. Comparatively, PPKTP demonstrates a larger temperature bandwidth and better temperature insensitivity than periodically poled lithium niobate (PPLN) in external cavity sum frequency mixing for laser generation, showcasing its potential advantages in practical applications. Additionally, the type-0 phase matching of PPKTP offers a larger acceptance angle and superior gain spectrum through temperature tuning or crystal rotation in optical parametric amplification processes.
Is there intravascular OCT working at 1060 nm?
5 answers
Yes, there is intravascular OCT working at 1060 nm. While one study introduces an integrated intravascular PT-OCT system based on spectral domain OCT, other research focuses on OCT systems operating at 1060 nm for imaging the human posterior eye segment, including the retina, choroid, and sclera. These systems utilize high-speed swept sources at 1060 nm, offering deep tissue penetration and low water absorption for in vivo imaging. Additionally, an OCT system is designed to switch between anterior and posterior segment imaging modes, with the posterior mode specifically utilizing light within the 1060 nm water absorption window. Overall, these studies highlight the feasibility and advancements in intravascular OCT systems operating at 1060 nm for various imaging applications.
How does machine learning and deep learning improve spectrum sensing in CR-IoT systems?
5 answers
Machine learning and deep learning techniques play a crucial role in enhancing spectrum sensing in Cognitive Radio-based Internet of Things (CR-IoT) systems. Deep learning architectures and image processing methods aid in improving CR systems' detection probability, maximizing the underutilized RF spectrum in 5G. However, the black-box nature of deep neural networks poses security risks, leading to the fusion of traditional interference methods and data poisoning attacks to reduce sensing accuracy in DL-based spectrum sensing systems. Cognitive radios, equipped with machine learning capabilities, can learn optimal spectrum utilization strategies, adapt operational settings, and identify idle channels efficiently, overcoming obstacles like spectrum sensing, management, security, and cross-layer design in CR networks. Additionally, integrating cognitive radios into IoT devices using hybrid spectrum access techniques and energy detectors for spectrum sensing can significantly enhance throughput, especially in low SNR environments.
How do theta waves influence episodic memory tasks?
5 answers
Theta waves play a crucial role in episodic memory tasks by aiding in the encoding and retrieval processes. Studies have shown that theta oscillations increase during successful memory encoding and before recall of previously learned items. Additionally, theta waves have been linked to the normalization of episodic memories relative to the density of sensory information encountered, suggesting that the human hippocampus adjusts the information content of memories based on sensory input. Furthermore, ongoing fluctuations in theta activity at the time of encoding have been found to impact later memory performance, indicating that prestimulus theta activity influences memory encoding. Overall, theta waves are essential for organizing neural activity, coordinating interactions across brain regions, and facilitating the discretization of continuous experiences into discrete episodic memories.
What are the papers on stacked intelligent metasurfaces on localisation and direction finding?
4 answers
The papers on stacked intelligent metasurfaces focusing on localization and direction finding include research on Receiving Reconfigurable Intelligent Surfaces (R-RISs) for 3D localization with cm-level accuracy, and X-band space-time modulated metasurfaces for direction finding through signal covariance matrix estimation and multiple signal classification (MUSIC). Additionally, the innovative technology of Stacked Intelligent Metasurfaces (SIM) is explored for holographic multiple-input multiple-output (HMIMO) communications, enabling efficient signal processing without excessive radio-frequency chains. The SIM-based HMIMO system leverages automatic transmit precoding and receiver combining, optimizing phase shifts for enhanced performance and capacity. These papers collectively showcase the potential of intelligent metasurfaces in advancing localization and direction finding applications in wireless communications.
What is Bandwidth?
5 answers
Bandwidth refers to the capacity of a telecommunication channel to transmit data effectively. In the realm of telecommunications, the concept of bandwidth has evolved due to market transitions, leading to the emergence of bandwidth as a commodity traded in markets. In the field of bolometric interferometry, bandwidth plays a crucial role in modifying the work principle of interferometers and affecting their sensitivity to cosmic microwave background fluctuations. The plenoptic function analysis highlights that the bandwidth of signals painted on surfaces impacts the characterization of the plenoptic function, with dependencies on surface characteristics. Additionally, simulations on nonlinear media suggest that angular dispersion can enhance bandwidth, particularly in certain configurations. Broadband access providers have also introduced innovative pricing schemes like "liquid bandwidth" to optimize service utilization and profitability.
Notional vales of rice?
5 answers
Rice holds significant notional values beyond its traditional role as a staple food. It is recognized for its medicinal properties, including being a key component in life-saving oral rehydration solutions (ORS) and Ayurvedic treatments for various ailments. Additionally, certain rice varieties like basmati and colored rices are rich in minerals, polyphenols, and antioxidants, contributing to their functional food status. In the agricultural realm, flooded rice cultivars have been studied for their competitiveness with red rice simulating genotypes, showcasing varying levels of suppression and growth under competition conditions. Furthermore, the Rice representation is utilized in engineering to describe band-pass electrical signals and quantum noise in laser oscillators, aiding in the design of optical communication systems.
How does fiber laser technology work in creating a chamber within glass structures?
5 answers
Fiber laser technology is utilized in creating chambers within glass structures through various methods. One approach involves using a fiber-fed laser-heated process to additively manufacture low-profile 2.5D glass structures. Another method employs fiber-optic Fabry-Perot interferometry to develop a glass-diaphragm microphone with a wheel-like structure on a thin glass sheet, enhancing sensitivity and directional response. Additionally, a passively Q-switched fiber laser system can control the birefringent effect in optic fibers in real-time, ensuring stable nano-second pulses with narrower widths. Furthermore, a novel annular chamber structure multi-wavelength fiber laser based on Brillouin effect generates multi-wavelength lasers with fixed frequency intervals through linear and non-linear gain mechanisms. These diverse applications showcase the versatility and precision of fiber laser technology in creating chambers within glass structures.