What is the protocol of tomato pollen protoplast isolation?
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The protocol for tomato pollen protoplast isolation involves several steps. First, the pollen grains are pre-treated at a low temperature for a specific duration. Then, the tetrads are transferred into an enzyme solution containing various components such as snailase, osmolites, cellulose, hemicellulase, pectolyase, sucrose, and other medium compositions. Among the different donor materials, the tetraploid variety Gannongshu No. 3 showed the highest protoplast yield. The isolated protoplasts can be cultured in a basic medium supplemented with specific growth factors to induce cell division and the formation of multicellular structures. This culture system allows for the isolation of generative cells, sperm cells, and vegetative cell nuclei from germinated tomato pollen grains and growing pollen tubes. The purity and viability of the isolated cells can be confirmed through microscopy examination and staining techniques.
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05 Jun 2017 | The provided paper does not mention the protocol for tomato pollen protoplast isolation. |
| The provided paper does not mention the protocol for tomato pollen protoplast isolation. The paper focuses on the effect of different sample preparation procedures for pollen isolation on the results of metabolic profiling. | |
20 Citations | The provided paper does not provide information about the protocol of tomato pollen protoplast isolation. |
4 Citations | The provided paper is about the isolation and culture of pollen tetrad protoplasts from Solanum tuberosum (potato). It does not provide information about the protocol for tomato pollen protoplast isolation. |
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