What is the purpose of Benedict's reagent?
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Benedict's reagent is used as a selective oxidizing agent to detect and quantify easily oxidized compounds . It is a modified version of the classical Benedict test, which is less sensitive. The reagent contains cupric ion (Cu2+), which is reduced to cuprous ion (Cu+) in the reaction. The presence of cuprous ion can be detected and quantified using 2,2'-bicinchoninic acid, which forms an intense purple complex with cuprous ion. The color development in the modified Benedict test depends on pH, temperature, and time. This improved method of the Benedict test has enhanced sensitivity and allows for the quantitation of compounds, making it useful for studying Benedict-positive compounds that are available only in small amounts .
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| Papers (5) | Insight |
|---|---|
Patent 06 Jul 2005 4 Citations | The purpose of Benedict's reagent is not mentioned in the provided information. |
Patent 11 Apr 1995 3 Citations | The purpose of Benedict's reagent is to test for the presence of reducing sugars in a solution. |
| The purpose of St. Benedict is to measure the beta-neutrino angular correlation parameter in superallowed mixed beta-decay transitions between mirror nuclei. | |
4 Citations | The purpose of Benedict's reagent is to detect and quantify compounds through the reduction of cupric ion to cuprous ion. |
| The purpose of Benedict's reagent is to detect easily oxidized compounds. |
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How can wound healing assay help determine effectiveness of a reagent?5 answersStep 1: Answer without citation
The wound healing assay can help determine the effectiveness of a reagent by creating a controlled environment to observe and measure cell migration in response to specific stimuli. Different methods, such as microfabricated 96-well assays and microfluidics-based assays, allow for high-throughput measurement of cellular migration capabilities. By interpreting the numbers of migrated cells into the wound regions as a function of specific stimuli with different concentrations, the half-maximal effective concentration (EC50) can be obtained, providing quantitative data on the effectiveness of the reagent. Additionally, the assay can be used to study the effects of physical or chemical stimuli on wound-healing speeds, providing insights into the mechanisms of the wound-healing process and the development of new wound-healing therapies.
Step 2: Finding Citations
- "Context_2" for the microfabricated 96-well wound-healing assay
- "Context_5" for the microfluidics-based wound-healing assay
Step 3: Answer with citation
The wound healing assay can help determine the effectiveness of a reagent by creating a controlled environment to observe and measure cell migration in response to specific stimuli. Different methods, such as microfabricated 96-well assays and microfluidics-based assays, allow for high-throughput measurement of cellular migration capabilities. By interpreting the numbers of migrated cells into the wound regions as a function of specific stimuli with different concentrations, the half-maximal effective concentration (EC50) can be obtained, providing quantitative data on the effectiveness of the reagent. Additionally, the assay can be used to study the effects of physical or chemical stimuli on wound-healing speeds, providing insights into the mechanisms of the wound-healing process and the development of new wound-healing therapies.
Advantages and disadvantages of benedict's method instead fehlings's method for detecting urinary reducing agents like glucose?3 answersBenedict's method has advantages and disadvantages compared to Fehling's method for detecting urinary reducing agents like glucose. One advantage of Benedict's method is that it can detect a wide range of reducing agents, including glucose, and provide qualitative results from negative to positive. This is shown in the study by Fitri Fadhilah and Noviana Vanawati. However, there are some disadvantages to using Benedict's method. The process of urine reduction examination using a flame requires a longer time and can be time-consuming, as it can only be done one sample at a time. There is also a risk of the solution in the tube exploding. On the other hand, Fehling's method, although not mentioned in the provided abstracts, is known to be more specific for glucose detection and can provide quantitative results. It is also less time-consuming and can be done with multiple samples simultaneously.
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