What is the sky?
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Spectral karyotyping (SKY) is a cytogenetic technique that allows for the visualization and identification of all 24 human chromosomes in a single experiment. It is used to detect chromosomal aberrations and rearrangements associated with cancer and genetic diseases. SKY is hyper accurate, hypersensitive, and hyper intuitionist, and can detect abnormalities that may be missed by other techniques such as conventional banding and fluorescence in situ hybridization (FISH) . SKY requires metaphase chromosomes, while FISH can be used on non-dividing cells at interphase. Both SKY and FISH have the advantage of generating information at the single cell level, which is valuable for studying complex processes such as cellular differentiation and disease progression . SKY has revolutionized cancer cytogenetics by providing a more detailed description of highly abnormal karyotypes and facilitating the identification of subtle structural rearrangements . SKY uses a combination of probe labeling, fluorescence microscopy, spectroscopy, CCD-imaging, and spectral image analysis to assign different colors to each chromosome, allowing for accurate analysis of abnormal karyotypes .
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53 Citations | The paper provides information about Spectral Karyotyping (SKY), which is a powerful 24-color, whole chromosome-painting assay that allows the visualization of each chromosome in one experiment. |
| The paper does not provide a specific definition of "sky". | |
7 Citations | SKY stands for Spectral karyotyping, a cytogenetic technique that can display and identify all 24 human chromosomes simultaneously without prior knowledge of any abnormalities involved. |
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