What specific genes were identified as unique for Brucella species?
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Unique genes identified for Brucella species include mprF, bepG, bepF, bepC, bepE, and bepD, associated with virulence factors, as found in all isolates from Iran . Additionally, a specific reverse complement sequence between B. canis and other Brucella species was discovered, leading to the development of a PCR method for species differentiation . Moreover, a study on antimicrobial susceptibility in Brucella isolates identified VecC and BetB genes in B. abortus as comparable, highlighting antibiotic resistance in Brucella spp. . Furthermore, a comparative genomic analysis revealed unique insertion sequence (IS) elements among different Brucella species, aiding in species differentiation through species-specific primers and multiplex PCR . These findings contribute significantly to understanding the genetic diversity and species-specific characteristics of Brucella pathogens.
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|---|---|
| The BCSP-31 gene of Brucella abortus was identified as unique, playing a significant role in marker and DNA vaccine development for cases of bovine abortion in India. | |
| A 132 bp reverse complement sequence between DK60_915 and DK60_917 loci in B. canis chromosome 1 was identified as unique for distinguishing Brucella canis from other Brucella species. | |
10 Citations | Unique insertion sequences (IS) were identified in Brucella species: 25 in B. abortus, 27 in B. melitensis, 37 in B. ovis, 86 in B. suis, and 3 in B. canis. |
| Unique genes identified for Brucella species were VecC and BetB, confirmed to be comparable between B. abortus strains, indicating potential antibiotic resistance reservoirs in South Africa. | |
| Unique genes identified in Brucella species were mprF, bepG, bepF, bepC, bepE, and bepD. These genes were found in all isolates, showcasing consistency across different strains. |
Related Questions
How does the genetic diversity of different animal species impact the spread and severity of brucella?5 answersThe genetic diversity of Brucella species in various animal populations plays a crucial role in the spread and severity of brucellosis. Studies from Turkey, Kazakhstan, Egypt, Costa Rica, and worldwide highlight the significance of genetic typing methods like MLVA-16 and whole-genome sequencing in understanding the epidemiology of Brucella strains. Different genotypes and lineages of B. melitensis and B. abortus have been identified, showing population dynamics and spread patterns across regions. The movement of infected animals, trade practices, and historical links between countries contribute to the dissemination of brucellosis. Genetic diversity changes over time, emphasizing the need for continuous surveillance to track the evolution of the pathogen and implement effective control measures to prevent human and animal suffering.
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Isolation of brucella abortus from soil sample5 answersBrucella abortus has been isolated from soil samples in multiple studies. In a study conducted in Isfahan, Iran, it was found that the banding pattern of PCR-RFLP for some isolates was similar to B. abortus, indicating the presence of B. abortus in the area. Another study conducted in South Moravia, Czech Republic, successfully isolated B. microti, a species closely related to B. abortus, from soil samples collected near mouse burrows. The isolates were confirmed to be B. microti through genetic analysis, and it was suggested that soil may serve as a reservoir for this species. Therefore, it is possible to isolate B. abortus from soil samples, and these findings highlight the importance of considering soil as a potential source of Brucella infections.
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What are the antigenic characteristics of brucella melitensis?4 answersBrucella melitensis has several antigenic characteristics. The outer membrane protein-31 (Omp31) is a major protein of Brucellae, except for B. abortus, and its antigenic epitopes have been characterized using monoclonal antibodies (mAbs). A total of 22 mAbs were produced against Omp31 of B. melitensis, and they recognized linear, semi-conformational, and conformational epitopes. Some of these mAbs reacted with conserved linear epitopes of B. melitensis, B. ovis, B. suis, and B. canis strains, while others highly reacted with a conformational epitope of Omp31. Additionally, a crude protein extract of B. melitensis was found to be immunogenic, with certain proteins showing higher immunogenicity. These findings suggest that Omp31 and other proteins of B. melitensis can be used for antigen detection and identification, as well as for developing diagnostic antibodies.
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